JCM Figure table search 04
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Aldeen, W. E.
Right arrow Articles by Hale, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Aldeen, W. E.
Right arrow Articles by Hale, D.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, May 1998, p. 1338-1340, Vol. 36, No. 5
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Comparison of Nine Commercially Available Enzyme-Linked Immunosorbent Assays for Detection of Giardia lamblia in Fecal Specimens

William E. Aldeen,1,* K. Carroll,2 A. Robison,2 M. Morrison,2 and D. Hale2

Associated Regional and University Pathologists, Inc., Salt Lake City, Utah 84108,1 and Department of Pathology, University of Utah Medical School, Salt Lake City, Utah 841322

Received 11 September 1997/Returned for modification 20 October 1997/Accepted 19 February 1998

Overall performance, including ease of use, total hands-on time, incubation and processing times, sensitivity, and specificity, of each of nine enzyme-linked immunosorbent assays (ELISAs) were compared by using 222 individual fecal samples submitted for the detection of Giardia lamblia. The assays evaluated were manufactured by Alexon, Inc., Cambridge Biotech Corp., Meridian, Inc., and Trend Scientific, Inc. All assays used polyclonal antibodies except the "new and improved" Microplate (direct and diluted methods) by Alexon, which is a monoclonal antibody assay. Seventy specimens were positive for G. lamblia by ELISA, ova and parasite test, and/or direct fluorescent-antibody assay. One hundred fifty two were negative by all three methods. Sensitivities and specificities ranged from 88.6 to 100% and 99.3 to 100%, respectively. The total hands-on time needed to run one specimen ranged from 1 min to 2 min 17 s per specimen. All except one commercially available ELISA were found to be rapid, sensitive, and specific for the detection of G. lamblia in fecal specimens.

* Corresponding author. Mailing address: ARUP, Inc., 500 Chipeta Way, Salt Lake City, UT 84108. Phone: (801) 583-2787, ext. 2726. Fax: (801) 583-2712. E-mail: SMTP:Aldeenwe{at}ARUP-Lab.com.

Journal of Clinical Microbiology, May 1998, p. 1338-1340, Vol. 36, No. 5
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 1998 by the American Society for Microbiology. All rights reserved.