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Journal of Clinical Microbiology, May 1998, p. 1378-1381, Vol. 36, No. 5
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Multicenter Comparison of ESP Culture System II with BACTEC 460TB and with Lowenstein-Jensen Medium for Recovery of Mycobacteria from Different Clinical Specimens, Including Blood

Enrico Tortoli,1,* Paola Cichero,2 M. Gabriella Chirillo,3 M. Rita Gismondo,4 Letizia Bono,5 Giampietro Gesu,2 M. Tullia Simonetti,1 Gisella Volpe,3 Giampietro Nardi,4 and Piero Marone5

Laboratorio di Microbiologia e Virologia, Ospedale di Careggi, 50139 Florence,1 Laboratorio di Microbiologia, H. S. Raffaele, 20132 Milan,2 Laboratorio di Microbiologia, Ospedale S. Luigi, 10043 Orbassano,3 Laboratorio di Microbiologia, Polo Universitario Ospedale Sacco, 20157 Milan,4 and Laboratori di Area Infettivologica, IRCCS S. Matteo, 27100 Pavia,5 Italy

Received 17 December 1997/Returned for modification 8 February 1998/Accepted 20 February 1998

The recently developed ESP Culture System II (AccuMed, Chicago, Ill.) was compared with radiometric BACTEC 460TB (Becton Dickinson, Towson, Md.) and with Lowenstein-Jensen medium for recovery of mycobacteria from over 2,500 clinical specimens both of respiratory and nonrespiratory origin, including blood. The majority of the 219 mycobacterial isolates (129) belonged to the Mycobacterium tuberculosis complex, followed by 37 isolates of the Mycobacterium avium complex (MAC) and 53 isolates of eight other mycobacterial species. Rates of recovery obtained with BACTEC, ESP, and Lowenstein-Jensen medium were 89, 79, and 64%, respectively, with such differences being statistically significant. Different media and systems appeared to behave differently when the more frequently detected organisms were considered: M. tuberculosis complex isolates grew better with BACTEC, and MAC isolates grew better with ESP. An analysis of the combinations of Lowenstein-Jensen medium with BACTEC and with ESP did not reveal significant differences in recovery rates. With regard to the times needed for the detection of positive cultures, they were significantly longer on Lowenstein-Jensen medium (average, 28 days) than with the remaining two systems, between which there was no difference (average, 18 days). We conclude, therefore, that the ESP system, when used in combination with a solid medium, performs as well as the thoroughly validated radiometric BACTEC system and offers the advantages of full automation and absence of radioisotopes.

* Corresponding author. Mailing address: Laboratorio di Microbiologia, viale Pieraccini 24, 50139 Florence, Italy. Phone: 39-55-4277343. Fax: 39-55-4223895. E-mail: tortoli{at}dada.it.

Journal of Clinical Microbiology, May 1998, p. 1378-1381, Vol. 36, No. 5
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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