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Journal of Clinical Microbiology, May 1998, p. 1410-1413, Vol. 36, No. 5
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Nonrandom Association of IS6110 and Mycobacterium tuberculosis: Implications for Molecular Epidemiological Studies

T. D. McHugh and S. H. Gillespie*

Department of Microbiology, Royal Free Hospital School of Medicine, London NW3 2PF, United Kingdom

Received 20 October 1997/Returned for modification 10 January 1998/Accepted 29 January 1998

IS6110 restriction fragment length polymorphism typing is now established as the primary typing method for Mycobacterium tuberculosis. It has been assumed that the position of bands is random. Thus, the discrimination of the technique increases in proportion to the copy number. Two collections of M. tuberculosis were investigated to test this hypothesis. We identified 33 positions in isolates from a Tanzanian collection and 25 positions in isolates from a London, United Kingdom, collection where bands were significantly more likely to be present than would be expected by chance. These data suggest that band position is not random, and this possibility may have an impact on the interpretation of molecular epidemiological studies of M. tuberculosis.

* Corresponding author. Mailing address: Department of Microbiology, Royal Free Hospital School of Medicine, Rowland Hill St., London NW3 2PF, United Kingdom. Phone: 44-171-794-0500. Fax: 44-171-794-0433. E-mail: stepheng{at}rfhsm.ac.uk.

Journal of Clinical Microbiology, May 1998, p. 1410-1413, Vol. 36, No. 5
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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