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Journal of Clinical Microbiology, May 1998, p. 1419-1421, Vol. 36, No. 5
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Apparent Increased Prevalence of High-Level Aminoglycoside-Resistant Enterococcus durans Resulting from False Identification by a Semiautomated Software System

Athanassios Tsakris,1,* Neil Woodford,2 Spyros Pournaras,3 Mary Kaufmann,2 and John Douboyas3

Department of Microbiology, Medical School, Aristotelian University of Thessaloniki,1 and Department of Microbiology, AHEPA University Hospital,3 Thessaloniki, Greece, and Laboratory of Hospital Infection, Central Public Health Laboratory, London, United Kingdom2

Received 31 October 1997/Returned for modification 2 January 1998/Accepted 6 February 1998

Identification of enterococci by using a semiautomated system (PASCO; Difco Laboratories, Detroit, Mich.) in the AHEPA University Hospital, Thessaloniki, Greece, revealed a high proportion of Enterococcus durans, particularly among isolates highly resistant to gentamicin and streptomycin. When 14 isolates were further tested by a conventional biochemical scheme and by PCR, all were reidentified as Enterococcus faecalis. Antibiotic resistance and pulsed-field gel electrophoresis patterns showed that unrelated strains were misidentified.

* Corresponding author. Mailing address: Department of Microbiology, Medical School, Aristotelian University of Thessaloniki, 54006 Thessaloniki, Greece. Phone: 30 31 999 091. Fax: 30 31 999 149. E-mail: atsakris{at}med.auth.gr.

Journal of Clinical Microbiology, May 1998, p. 1419-1421, Vol. 36, No. 5
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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Copyright © 1998 by the American Society for Microbiology. All rights reserved.