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Journal of Clinical Microbiology, May 1998, p. 1428-1429, Vol. 36, No. 5
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Simplification of a Locus-Specific DNA Typing Method (Vir Typing) for Streptococcus pyogenes

J. Hartas,* M. Hibble, and K. S. Sriprakash

Menzies School of Health Research, Darwin, Australia

Received 25 November 1997/Returned for modification 22 January 1998/Accepted 4 February 1998

We describe a simplification of a highly discriminatory molecular typing method, called Vir typing, for Streptococcus pyogenes (D. Gardiner, J. Hartas, B. Currie, J. D. Mathews, D. J. Kemp, and K. S. Sriprakash, PCR Methods Appl. 4:288-293, 1995). The procedure can be completed within a day, is reproducible, and can be applied directly to colonies growing on primary culture plates, allowing rapid establishment of strain identity in an outbreak.

* Corresponding author. Mailing address: Menzies School of Health Research, P.O. Box 41096, Causarina NT 0811, Australia. Phone: 61-8-89228599. Fax: 61-8-89275187. E-mail: jon{at}menzies.su.edu.au.

Journal of Clinical Microbiology, May 1998, p. 1428-1429, Vol. 36, No. 5
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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