Comparison of a 2,3-Bis(2-Methoxy-4-Nitro-5-Sulfophenyl)-5-[(Phenylamino)Carbonyl]-2H-Tetrazolium Hydroxide (XTT) Colorimetric Method with the Standardized National Committee for Clinical Laboratory Standards Method of Testing Clinical Yeast Isolates for Susceptibility to Antifungal Agents

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Journal of Clinical Microbiology, May 1998, p. 1450-1452, Vol. 36, No. 5
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Comparison of a 2,3-Bis(2-Methoxy-4-Nitro-5-Sulfophenyl)-5-[(Phenylamino)Carbonyl]-2H-Tetrazolium Hydroxide (XTT) Colorimetric Method with the Standardized National Committee for Clinical Laboratory Standards Method of Testing Clinical Yeast Isolates for Susceptibility to Antifungal Agents

S. P. Hawser,¹^,^* H. Norris,² C. J. Jessup,² and M. A. Ghannoum²

Hoechst Marion Roussel, F-93235 Romainville, France,¹ and Mycology Reference Laboratory, Center for Medical Mycology, University Hospitals of Cleveland, and Department of Dermatology, Case Western Reserve University, Cleveland, Ohio 44106-5028²

Received 4 December 1997/Returned for modification 5 January 1998/Accepted 31 January 1998

MICs for clinical Candida and Cryptococcus isolates were determined by a method incorporating the colorimetric indicator 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazoliumhydroxide (XTT), and the results were compared with MICs obtainedby the National Committee for Clinical Laboratory Standards approvedstandard method (M27-A). One hundred percent of all isolates demonstratedagreement within 2 dilutions between the MICs of amphotericinB, fluconazole, itraconazole, ketoconazole, and flucytosine obtainedby the two methods. These data suggest that an XTT-based methodcould provide a useful means for the determination of antifungalsusceptibility of yeasts.

^* Corresponding author. Mailing address: Hoechst Marion Roussel, 102 Route de Noisy, F-93235 Romainville, France. Phone: 33-1-4991-3097.Fax: 33-1-4991-3116.

Journal of Clinical Microbiology, May 1998, p. 1450-1452, Vol. 36, No. 5
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

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