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Journal of Clinical Microbiology, May 1998, p. 1458-1460, Vol. 36, No. 5
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Rapid Detection of Species of the Opportunistic Yeast Trichosporon by PCR

Takashi Sugita, Akemi Nishikawa, and Takako Shinoda*

Department of Microbiology, Meiji College of Pharmacy, Tanashi, Tokyo, Japan

Received 4 August 1997/Returned for modification 7 October 1997/Accepted 30 January 1998

Trichosporon species are opportunistic pathogens, associated with a high mortality rate in immunocompromised patients. Oligonucleotide primers were used to amplify a 170-bp fragment of small-subunit ribosomal DNA of all species in the genus Trichosporon by PCR. The primers amplify DNAs of all species in the genus Trichosporon, including six causative agents of trichosporonosis. DNAs of other medically important yeasts, such as Candida albicans and Cryptococcus neoformans, are not amplified by this detection system.

* Corresponding author. Mailing address: Department of Microbiology, Meiji College of Pharmacy, 1-22-1 Yato-cho, Tanashi, Tokyo 188-0001 Japan. Phone: 81-424-21-0339. Fax: 81-424-21-1489. E-mail: shinoda{at}my-pharm.ac.jp.

Journal of Clinical Microbiology, May 1998, p. 1458-1460, Vol. 36, No. 5
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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