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Journal of Clinical Microbiology, May 1998, p. 1461-1463, Vol. 36, No. 5
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Comparison of DNA Enzyme Immunoassay and Line Probe Assays (Inno-LiPA HCV I and II) for Hepatitis C Virus Genotyping

S. Le Pogam,1 F. Dubois,1,2 R. Christen,3 C. Raby,1 A. Cavicchini,4 and A. Goudeau1,*

Département de Microbiologie Médicale et Moléculaire, CNRS EP 117-CHU Bretonneau, 37044 Tours Cedex,1 Institut Régional pour la Santé, 37521 La Riche Cedex,2 and Observatoire Océanologique-Station Zoologique, CNRS and Université Pierre et Marie Curie, 06230 Villefranche-sur-Mer Cedex,3 France, and Diagnostics Division, SORIN Biomedica, 13040 Saluggia (VC), Italy4

Received 28 October 1997/Returned for modification 22 December 1997/Accepted 9 February 1998

Two methods for genotyping hepatitis C virus (DNA enzyme immunoassay [DEIA] and line probe assay [Inno-LiPA HCV I and II]) were compared on 120 samples and of these 87% were assigned to the same subtype by both assays. There were 15 subtyping discrepancies which involved 5% of type 1 isolates and 90% of type 2 isolates. Amplified products from the core and 5' untranslated regions (UTR) were sequenced to resolve conflicts. Type 1 discordant samples had a guanosine at position -99 in the 5' UTR, a characteristic of genotype 1b, and a core region typical of subtype 1a. The eight isolates classified as 2a/2c by LiPA and as subtype 2c by DEIA belonged to type 2.

* Corresponding author. Mailing address: Département de Microbiologie Médicale et Moléculaire CNRS EP 117-CHU Bretonneau, 2 Boulevard Tonnellé, 37044 Tours Cedex, France. Phone: (33) 2 47 47 69 97. Fax: (33) 2 47 47 36 10. E-mail: gondeau{at}med.univ-tours.fr.

Journal of Clinical Microbiology, May 1998, p. 1461-1463, Vol. 36, No. 5
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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