This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chazalet, V.
Right arrow Articles by Latgé, J.-P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chazalet, V.
Right arrow Articles by Latgé, J.-P.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, June 1998, p. 1494-1500, Vol. 36, No. 6
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Molecular Typing of Environmental and Patient Isolates of Aspergillus fumigatus from Various Hospital Settings

Valérie Chazalet,1 Jean-Paul Debeaupuis,1 Jacqueline Sarfati,1 Jacques Lortholary,2 Patricia Ribaud,3 Pramod Shah,4 Muriel Cornet,5 Hoang Vu Thien,6 Eliane Gluckman,3 Gilles Brücker,2 and Jean-Paul Latgé1,*

Laboratoire des Aspergillus, Institut Pasteur, 75015 Paris,1 Assistance Publique Hôpitaux de Paris, SEHP, 75001 Paris,2 Unité de Greffe de Moelle Osseuse, Hôpital St Louis, 75010 Paris,3 Laboratoire de Microbiologie, Hôtel Dieu, 75004 Paris,5 and Laboratoire de Microbiologie, Hôpital Trousseau, 75012 Paris,6 France, and ZIM-Infektiologie, Universitäts Klinikum, 60590 Frankfurt, Germany4

Received 20 November 1997/Returned for modification 15 January 1998/Accepted 9 March 1998

Fingerprinting of more than 700 clinical and environmental isolates of Aspergillus fumigatus from four differential hospital settings was undertaken with a dispersed repeated DNA sequence. The analysis of the environmental isolates showed that the airborne A. fumigatus population is extremely diverse, with 85% of the strains being represented as a single genotype isolated once. The remaining 15% of the strains were isolated several times and were able to persist for several months in the same hospital environment. No strains were found to be associated with a specific location inside the hospital, and identical strains were isolated from different buildings of the hospital and outdoors. Isolation of the same strain both from patients and from the environment of the same hospital is highly suggestive of a nosocomial infection. The characteristics of the environmental fungal population explains the two main results obtained from the typing of the clinical isolates: (i) the absence of a common strain responsible for an invasive aspergillosis outbreak results from the extreme diversity of the environmental population of A. fumigatus in contact with the patients, and (ii) patients hospitalized in different wards of the same hospital can be infected with the same strain since every patient might inhale the same spore population.

* Corresponding author. Mailing address: Laboratoire des Aspergillus, Institut Pasteur, 25, rue du Dr Roux, 75724 Paris, France. Phone: 33-01-45-68-82-25. Fax: 33-01-40-61-34-19. E-mail: jplatge{at}pasteur.fr.

Journal of Clinical Microbiology, June 1998, p. 1494-1500, Vol. 36, No. 6
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Maschmeyer, G., Neuburger, S., Fritz, L., Bohme, A., Penack, O., Schwerdtfeger, R., Buchheidt, D., Ludwig, W.-D., on behalf of the Infectious Diseases Working Party, (2009). A prospective, randomised study on the use of well-fitting masks for prevention of invasive aspergillosis in high-risk patients. Ann Oncol 20: 1560-1564 [Abstract] [Full Text]  
  • Balajee, S. A., Tay, S. T., Lasker, B. A., Hurst, S. F., Rooney, A. P. (2007). Characterization of a Novel Gene for Strain Typing Reveals Substructuring of Aspergillus fumigatus across North America. Eukaryot Cell 6: 1392-1399 [Abstract] [Full Text]  
  • de Valk, H. A., Meis, J. F. G. M., de Pauw, B. E., Donnelly, P. J., Klaassen, C. H. W. (2007). Comparison of Two Highly Discriminatory Molecular Fingerprinting Assays for Analysis of Multiple Aspergillus fumigatus Isolates from Patients with Invasive Aspergillosis. J. Clin. Microbiol. 45: 1415-1419 [Abstract] [Full Text]  
  • Bain, J. M., Tavanti, A., Davidson, A. D., Jacobsen, M. D., Shaw, D., Gow, N. A. R., Odds, F. C. (2007). Multilocus Sequence Typing of the Pathogenic Fungus Aspergillus fumigatus. J. Clin. Microbiol. 45: 1469-1477 [Abstract] [Full Text]  
  • Balajee, S. A., Nickle, D., Varga, J., Marr, K. A. (2006). Molecular Studies Reveal Frequent Misidentification of Aspergillus fumigatus by Morphotyping.. Eukaryot Cell 5: 1705-1712 [Abstract] [Full Text]  
  • Chen, J., Li, H., Li, R., Bu, D., Wan, Z. (2005). Mutations in the cyp51A gene and susceptibility to itraconazole in Aspergillus fumigatus serially isolated from a patient with lung aspergilloma. J Antimicrob Chemother 55: 31-37 [Abstract] [Full Text]  
  • Healy, M., Reece, K., Walton, D., Huong, J., Shah, K., Kontoyiannis, D. P. (2004). Identification to the Species Level and Differentiation between Strains of Aspergillus Clinical Isolates by Automated Repetitive-Sequence-Based PCR. J. Clin. Microbiol. 42: 4016-4024 [Abstract] [Full Text]  
  • Jin, J., Lee, Y.-K., Wickes, B. L. (2004). Simple Chemical Extraction Method for DNA Isolation from Aspergillus fumigatus and Other Aspergillus Species. J. Clin. Microbiol. 42: 4293-4296 [Abstract] [Full Text]  
  • Warris, A., Klaassen, C. H. W., Meis, J. F. G. M., de Ruiter, M. T., de Valk, H. A., Abrahamsen, T. G., Gaustad, P., Verweij, P. E. (2003). Molecular Epidemiology of Aspergillus fumigatus Isolates Recovered from Water, Air, and Patients Shows Two Clusters of Genetically Distinct Strains. J. Clin. Microbiol. 41: 4101-4106 [Abstract] [Full Text]  
  • Lair-Fulleringer, S., Guillot, J., Desterke, C., Seguin, D., Warin, S., Bezille, A., Chermette, R., Bretagne, S. (2003). Differentiation between Isolates of Aspergillus fumigatus from Breeding Turkeys and Their Environment by Genotyping with Microsatellite Markers. J. Clin. Microbiol. 41: 1798-1800 [Abstract] [Full Text]  
  • Lasker, B. A. (2002). Evaluation of Performance of Four Genotypic Methods for Studying the Genetic Epidemiology of Aspergillus fumigatus Isolates. J. Clin. Microbiol. 40: 2886-2892 [Abstract] [Full Text]  
  • Bart-Delabesse, E., Sarfati, J., Debeaupuis, J.-P., van Leeuwen, W., van Belkum, A., Bretagne, S., Latge, J.-P. (2001). Comparison of Restriction Fragment Length Polymorphism, Microsatellite Length Polymorphism, and Random Amplification of Polymorphic DNA Analyses for Fingerprinting Aspergillus fumigatus Isolates. J. Clin. Microbiol. 39: 2683-2686 [Abstract] [Full Text]  
  • Zhao, J., Kong, F., Li, R., Wang, X., Wan, Z., Wang, D. (2001). Identification of Aspergillus fumigatus and Related Species by Nested PCR Targeting Ribosomal DNA Internal Transcribed Spacer Regions. J. Clin. Microbiol. 39: 2261-2266 [Abstract] [Full Text]  
  • Bertout, S., Renaud, F., Barton, R., Symoens, F., Burnod, J., Piens, M.-A., Lebeau, B., Viviani, M.-A., Chapuis, F., Bastide, J.-M., Grillot, R., Mallié, M., THE EUROPEAN RESEARCH GROUP ON BIOTYPE AND GENOTYP, (2001). Genetic Polymorphism of Aspergillus fumigatus in Clinical Samples from Patients with Invasive Aspergillosis: Investigation Using Multiple Typing Methods. J. Clin. Microbiol. 39: 1731-1737 [Abstract] [Full Text]  
  • CIMON, B., SYMOENS, F., ZOUHAIR, R., CHABASSE, D., NOLARD, N., DEFONTAINE, A., BOUCHARA, J.-P. (2001). Molecular epidemiology of airway colonisation by Aspergillus fumigatus in cystic fibrosis patients. J Med Microbiol 50: 367-374 [Abstract] [Full Text]  
  • Dannaoui, E., Borel, E., Monier, M.-F., Piens, M.-A., Picot, S., Persat, F. (2001). Acquired itraconazole resistance in Aspergillus fumigatus. J Antimicrob Chemother 47: 333-340 [Abstract] [Full Text]  
  • James, M. J., Lasker, B. A., McNeil, M. M., Shelton, M., Warnock, D. W., Reiss, E. (2000). Use of a Repetitive DNA Probe To Type Clinical and Environmental Isolates of Aspergillus flavus from a Cluster of Cutaneous Infections in a Neonatal Intensive Care Unit. J. Clin. Microbiol. 38: 3612-3618 [Abstract] [Full Text]  
  • Soll, D. R. (2000). The Ins and Outs of DNA Fingerprinting the Infectious Fungi. Clin. Microbiol. Rev. 13: 332-370 [Abstract] [Full Text]  
  • BERTOUT, S., RENAUD, F., DE MEEUS, T., PIENS, M.-A., LEBEAU, B., VIVIANI, M.-A., MALLIE, M., BASTIDE, J.-M. (2000). Multilocus enzyme electrophoresis analysis of Aspergillus fumigatus strains isolated from the first clinical sample from patients with invasive aspergillosis. J Med Microbiol 49: 375-381 [Abstract] [Full Text]  
  • Latge, J.-P. (1999). Aspergillus fumigatus and Aspergillosis. Clin. Microbiol. Rev. 12: 310-350 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»