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Journal of Clinical Microbiology, June 1998, p. 1568-1573, Vol. 36, No. 6
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Flow Cytometric Testing of Susceptibilities of Mycobacterium tuberculosis Isolates to Ethambutol, Isoniazid, and Rifampin in 24 Hours

Scott M. Kirk,1,2 Ronald F. Schell,2,3,4,* Andrea V. Moore,2,3 Steven M. Callister,5,6 and Gerald H. Mazurek7

Wisconsin State Laboratory of Hygiene3 and Departments of Medical Microbiology and Immunology2 and Bacteriology,4 University of Wisconsin, Madison, Wisconsin 53706; Microbiology Research Laboratory5 and Department of Infectious Diseases,6 Gundersen Lutheran Medical Center, LaCrosse, Wisconsin 54601; Bio-Rad Laboratories, Hercules, California 945471; and the Centers for Disease Control and Prevention, Atlanta, Georgia 303337

Received 7 November 1997/Returned for modification 22 December 1997/Accepted 24 February 1998

Susceptibility testing of Mycobacterium tuberculosis is seriously limited by the time required to obtain results. We show that susceptibility testing of clinical isolates of M. tuberculosis can be accomplished rapidly with acceptable accuracy by using flow cytometry. The susceptibilities of 35 clinical isolates of M. tuberculosis to various concentrations of isoniazid, rifampin, and ethambutol were tested by the agar proportion method and by flow cytometry. Agreement between the results from the two methods was 95, 92, and 83% for isoniazid, ethambutol, and rifampin, respectively. Only 11 discrepancies were detected among 155 total tests. The results of flow cytometric susceptibility tests were available within 24 h of inoculation of drug-containing medium, while the proportion method required 3 weeks to complete. The flow cytometric method is also simple to perform.

* Corresponding author. Mailing address: Wisconsin State Laboratory of Hygiene, University of Wisconsin, 465 Henry Mall, Madison, WI 53706. Phone: (608) 262-3634. Fax: (608) 265-3451.

Journal of Clinical Microbiology, June 1998, p. 1568-1573, Vol. 36, No. 6
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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