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Journal of Clinical Microbiology, June 1998, p. 1584-1587, Vol. 36, No. 6
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Use of Tests for Acidification of Methyl-alpha -D-Glucopyranoside and Susceptibility to Efrotomycin for Differentiation of Strains of Enterococcus and Some Related Genera

Maria Da Glória S. Carvalho,1,2 Lúcia M. Teixeira,1,2 and Richard R. Facklam2,*

Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941, Brazil,1 and Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 303332

Received 10 November 1997/Returned for modification 20 January 1998/Accepted 24 February 1998

A total of 107 Enterococcus strains, 10 Vagococcus fluvialis strains, and 8 Lactococcus garvieae strains were tested for acidification of methyl-alpha -D-glucopyranoside (MGP) and susceptibility to 100-µg efrotomycin (EFRO) disks. All 26 strains of Enterococcus casseliflavus, including 3 nonmotile and 2 nonpigmented strains, acidified MGP and were resistant to EFRO. All 22 strains of Enterococcus gallinarum, including 5 nonmotile strains, also acidified MGP and were resistant to EFRO. None of the 26 strains of Enterococcus faecium acidified MGP, and all were susceptible to EFRO. Although all 12 Enterococcus faecalis strains were also negative in the MGP test, they were resistant to EFRO. Other enterococcal strains gave variable results. All 10 strains of V. fluvialis and all 8 strains of L. garvieae gave positive and negative results, respectively, in the MGP test and were, respectively, resistant and susceptible to EFRO. These results indicate that tests of the production of acid from MGP and susceptibility to EFRO can be used as adjunct tests in the identification of typical and atypical strains of enterococci in the clinical microbiology laboratory.

* Corresponding author. Mailing address: Childhood and Respiratory Diseases Branch MS-C02, Centers for Disease Control and Prevention, Atlanta, GA 30333. Phone: (404) 639-1379. Fax: (404) 639-3123. E-mail: rrf2{at}cdc.gov.

Journal of Clinical Microbiology, June 1998, p. 1584-1587, Vol. 36, No. 6
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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