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Journal of Clinical Microbiology, June 1998, p. 1688-1692, Vol. 36, No. 6
Instituto de Biotecnología,
Received 10 November 1997/Returned for modification 2 February
1998/Accepted 11 March 1998
In the present investigation we characterized the antigenic
diversity of the VP4 and VP7 proteins in 309 and 261 human rotavirus strains isolated during two consecutive epidemic seasons, respectively, in three different regions of Mexico. G3 was found to be the prevalent VP7 serotype during the first year, being superseded by serotype G1
strains during the second season. To antigenically characterize the VP4
protein of the strains isolated, we used five neutralizing monoclonal
antibodies (MAbs) which showed specificity for VP4 serotypes P1A, P1B,
and P2 in earlier studies. Eight different patterns of reactivity with
these MAbs were found, and the prevalence of three of these patterns
varied from one season to the next. The P genotype of a subset of 52 samples was determined by PCR. Among the strains characterized as
genotype P[4] and P[8] there were three and five different VP4 MAb
reactivity patterns, respectively, indicating that the diversity of
neutralization epitopes in VP4 is greater than that previously
appreciated by the genomic typing methods.
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Antigenic and Genomic Diversity of Human Rotavirus VP4 in Two
Consecutive Epidemic Seasons in Mexico

*
Corresponding author. Mailing address: Instituto de
Biotecnología/UNAM, A.P. 510-3, Colonia Miraval, Cuernavaca,
Morelos 62250, México. Phone: (52-73) 29-1661. Fax: (52-73)
17-2388. E-mail: arias{at}ibt.unam.mx.
Present address: Departamento de Biología Molecular,
Instituto de Investigaciones Biomédicas, Universidad Nacional
Autónoma de México, Apdo. Postal 70-228, Mexico City 04510, Mexico.
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