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Journal of Clinical Microbiology, June 1998, p. 1688-1692, Vol. 36, No. 6
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Antigenic and Genomic Diversity of Human Rotavirus VP4 in Two Consecutive Epidemic Seasons in Mexico

Luis Padilla-Noriega,1,dagger Martha Méndez-Toss,1 Griselda Menchaca,2 Juan F. Contreras,2 Pedro Romero-Guido,1 Fernando I. Puerto,3 Héctor Guiscafré,4 Felipe Mota,5 Ismael Herrera,6 Roberto Cedillo,4 Onofre Muñoz,4 Juan Calva,7 María de Lourdes Guerrero,7 Barbara S. Coulson,8 Harry B. Greenberg,9 Susana López,1 and Carlos F. Arias1,*

Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos,1 Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Monterrey, Nuevo León,2 Centro de Investigaciones Regionales "Hideyo Noguchi," Universidad Autónoma de Yucatán, Mérida, Yucatán,3 Instituto Mexicano del Seguro Social4 and Unidad de Rehidratación Oral, Hospital Infantil de México,5 Mexico City, Facultad de Medicina, Universidad Autónoma de San Luis Potosí, San Luis Potosí, San Luis Potosí,6 and Instituto Nacional de la Nutrición Salvador Zubirán,7 Mexico; University of Melbourne, Parkville, Victoria, Australia8 and Stanford University, Stanford, California9

Received 10 November 1997/Returned for modification 2 February 1998/Accepted 11 March 1998

In the present investigation we characterized the antigenic diversity of the VP4 and VP7 proteins in 309 and 261 human rotavirus strains isolated during two consecutive epidemic seasons, respectively, in three different regions of Mexico. G3 was found to be the prevalent VP7 serotype during the first year, being superseded by serotype G1 strains during the second season. To antigenically characterize the VP4 protein of the strains isolated, we used five neutralizing monoclonal antibodies (MAbs) which showed specificity for VP4 serotypes P1A, P1B, and P2 in earlier studies. Eight different patterns of reactivity with these MAbs were found, and the prevalence of three of these patterns varied from one season to the next. The P genotype of a subset of 52 samples was determined by PCR. Among the strains characterized as genotype P[4] and P[8] there were three and five different VP4 MAb reactivity patterns, respectively, indicating that the diversity of neutralization epitopes in VP4 is greater than that previously appreciated by the genomic typing methods.

* Corresponding author. Mailing address: Instituto de Biotecnología/UNAM, A.P. 510-3, Colonia Miraval, Cuernavaca, Morelos 62250, México. Phone: (52-73) 29-1661. Fax: (52-73) 17-2388. E-mail: arias{at}ibt.unam.mx.

dagger Present address: Departamento de Biología Molecular, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Apdo. Postal 70-228, Mexico City 04510, Mexico.

Journal of Clinical Microbiology, June 1998, p. 1688-1692, Vol. 36, No. 6
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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Copyright © 1998 by the American Society for Microbiology. All rights reserved.