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Journal of Clinical Microbiology, June 1998, p. 1733-1736, Vol. 36, No. 6
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Geographic Discrimination of Paracoccidioides brasiliensis Strains by Randomly Amplified Polymorphic DNA Analysis

Ana María Calcagno, Gustavo Niño-Vega, Felipe San-Blas, and Gioconda San-Blas*

Instituto Venezolano de Investigaciones Científicas (IVIC), Centro de Microbiología y Biología Celular, Laboratorio de Micología, Caracas 1020A, Venezuela

Received 10 October 1997/Returned for modification 24 November 1997/Accepted 19 March 1998

Randomly amplified polymorphic DNA (RAPD) analysis of 33 Paracoccidioides brasiliensis strains from Argentina, Brazil, Colombia, Peru, and Venezuela produced reproducible amplification products which were sufficiently polymorphic to allow differentiation of the strains. Types generated with five primers (OPG 03, OPG 05, OPG 14, OPG 16, and OPG 18) resulted in a high discriminatory index (0.956). The discriminatory index was slightly reduced (0.940) when only two primers (OPG 3 and OPG 14) were used. A dendrogram based on these results showed a high degree of similarity among the strains, and genetic differences were expressed in clusters related to geographical regions but not to pathological features of the disease. With a few exceptions, strains were sorted into five groups by geographical origin as follows: group I, Venezuelan strains; group II, Brazilian strains; group III, Peruvian strains; group IV, Colombian strains; and group V, Argentinian strains. The group containing the most disparate strains was group V (discriminatory index, 0.633); the discriminatory index for the other four groups was 0.824. The use of primer OPG 18 by itself was sufficient to discriminate species specificity, and the use of primer OPG 14 by itself was sufficient to discriminate among the geographical locations of the strains in the sample. This method may be helpful for epidemiological studies of P. brasiliensis.

* Corresponding author. Mailing address: Centro de Microbiología y Biología Celular, Laboratorio de Micología, Apartado 21827, Caracas 1020A, Venezuela. Phone: 58-2-504 1496. Fax: 58-2-504 1382. E-mail: gsanblas{at}pasteur.ivic.ve.

Journal of Clinical Microbiology, June 1998, p. 1733-1736, Vol. 36, No. 6
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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