This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by O'Rourke, K. I.
Right arrow Articles by Knowles, D. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by O'Rourke, K. I.
Right arrow Articles by Knowles, D. P.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, June 1998, p. 1750-1755, Vol. 36, No. 6
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Monoclonal Antibody F89/160.1.5 Defines a Conserved Epitope on the Ruminant Prion Protein

Katherine I. O'Rourke,1,* Timothy V. Baszler,2,3 Janice M. Miller,4 Terry R. Spraker,5 Ingrid Sadler-Riggleman,1,dagger and Donald P. Knowles1

Animal Disease Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Pullman, Washington 99164-70301; Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-70402; Washington Animal Disease Diagnostic Laboratory, Washington State University, Pullman, Washington 991643; National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa 500104; and Colorado State Diagnostic Laboratory, College of Veterinary Medicine, Colorado State University, Fort Collins, Colorado 805235

Received 19 August 1997/Returned for modification 11 December 1997/Accepted 27 January 1998

The transmissible spongiform encephalopathies are a heterogeneous group of fatal neurodegenerative disorders occurring in humans, mink, cats, and ruminant herbivores. The occurrence of novel transmissible spongiform encephalopathies in cattle in the United Kingdom and Europe and in mule deer and elk in parts of the United States has emphasized the need for reliable diagnostic tests with standardized reagents. Postmortem diagnosis is performed by histologic examination of brain sections from affected animals. The histopathological criteria for transmissible spongiform encephalopathies include gliosis, astrocytosis, neuronal degeneration, and spongiform change. These lesions vary in intensity and anatomic location depending on the host species and genetics, stage of disease, and infectious agent source. Diagnosis by histopathology alone may be ambiguous in hosts with early cases of disease and impossible if the tissue is autolyzed. Deposition of the prion protein (an abnormal isoform of a native cellular sialoglycoprotein) in the central nervous system is a reliable marker for infection, and immunohistochemical detection of this marker is a useful adjunct to histopathology. In the present paper we describe monoclonal antibody (MAb) F89/160.1.5, which reacts with prion protein in tissues from sheep, cattle, mule deer, and elk with naturally occurring transmissible spongiform encephalopathies. This MAb recognizes a conserved epitope on the prion protein in formalin-fixed, paraffin-embedded sections after hydrated autoclaving. MAb F89/160.1.5 will be useful in diagnostic and pathogenesis studies of the transmissible spongiform encephalopathies in these ruminant species.

* Corresponding author. Mailing address: Animal Disease Research Unit, Agricultural Research Service, U.S. Department of Agriculture, 337 Bustad Hall, WSU, Pullman, WA 99164-7030. Phone: (509) 335-6020. Fax: (509) 335-8328. E-mail: korourke{at}vetmed.wsu.edu.

dagger Present address: 560 Quail Ridge, Pullman, WA 99164.

Journal of Clinical Microbiology, June 1998, p. 1750-1755, Vol. 36, No. 6
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Hamir, A. N., Richt, J. A., Kunkle, R. A., Greenlee, J. J., Bulgin, M. S., Gregori, L., Rohwer, R. G. (2009). Characterization of a US Sheep Scrapie Isolate with Short Incubation Time. Vet Pathol 46: 1205-1212 [Abstract] [Full Text]  
  • Smith, J. D., Greenlee, J. J., Hamir, A. N., Richt, J. A., Greenlee, M. H.W. (2009). Retinal Function and Morphology Are Altered in Cattle Infected with the Prion Disease Transmissible Mink Encephalopathy. Vet Pathol 46: 810-818 [Abstract] [Full Text]  
  • Smith, J. D., Greenlee, J. J., Hamir, A. N., Greenlee, M. H. W. (2009). Altered electroretinogram b-wave in a Suffolk sheep experimentally infected with scrapie. Vet Rec. 165: 179-181 [Full Text]  
  • Hamir, A. N., Kunkle, R. A., Greenlee, J. J., Richt, J. A. (2009). Experimental oral transmission of United States origin scrapie to neonatal sheep. jvdi 21: 64-68 [Abstract] [Full Text]  
  • Hamir, A. N., Kunkle, R. A., Richt, J. A., Greenlee, J. J., Miller, J. M. (2009). Serial Passage of Sheep Scrapie Inoculum in Suffolk Sheep. Vet Pathol 46: 39-44 [Abstract] [Full Text]  
  • Green, K. M., Browning, S. R., Seward, T. S., Jewell, J. E., Ross, D. L., Green, M. A., Williams, E. S., Hoover, E. A., Telling, G. C. (2008). The elk PRNP codon 132 polymorphism controls cervid and scrapie prion propagation. J. Gen. Virol. 89: 598-608 [Abstract] [Full Text]  
  • Hamir, A. N., Kunkle, R. A., Richt, J. A., Miller, J. M., Greenlee, J. J. (2008). Experimental Transmission of US Scrapie Agent by Nasal, Peritoneal, and Conjunctival Routes to Genetically Susceptible Sheep. Vet Pathol 45: 7-11 [Abstract] [Full Text]  
  • Seabury, C. M., Gill, C. A., Templeton, J. W., Dyar, J. B., Derr, J. N., Adelson, D. L., Owens, E., Davis, D. S., Kraemer, D. C., Womack, J. E. (2007). Molecular Characterization of the Rocky Mountain Elk (Cervus elaphus nelsoni) PRNP Putative Promoter. J Hered 0: esm091v1-esm091 [Abstract] [Full Text]  
  • Spinner, D. S., Kascsak, R. B., LaFauci, G., Meeker, H. C., Ye, X., Flory, M. J., Kim, J. I., Schuller-Levis, G. B., Levis, W. R., Wisniewski, T., Carp, R. I., Kascsak, R. J. (2007). CpG oligodeoxynucleotide-enhanced humoral immune response and production of antibodies to prion protein PrPSc in mice immunized with 139A scrapie-associated fibrils. J. Leukoc. Biol. 81: 1374-1385 [Abstract] [Full Text]  
  • Gretzschel, A., Buschmann, A., Langeveld, J., Groschup, M. H. (2006). Immunological characterization of abnormal prion protein from atypical scrapie cases in sheep using a panel of monoclonal antibodies. J. Gen. Virol. 87: 3715-3722 [Abstract] [Full Text]  
  • Georgsson, G., Sigurdarson, S., Brown, P. (2006). Infectious agent of sheep scrapie may persist in the environment for at least 16 years. J. Gen. Virol. 87: 3737-3740 [Abstract] [Full Text]  
  • Hamir, A. N., Kunkle, R. A., Cutlip, R. C., Miller, J. M., Williams, E. S., Richt, J. A. (2006). Transmission of chronic wasting disease of mule deer to Suffolk sheep following intracerebral inoculation. jvdi 18: 558-565 [Abstract] [Full Text]  
  • Greenlee, J. J., Hamir, A. N., West Greenlee, M. H. (2006). Abnormal prion accumulation associated with retinal pathology in experimentally inoculated scrapie-affected sheep.. Vet Pathol 43: 733-739 [Abstract] [Full Text]  
  • Klingeborn, M., Wik, L., Simonsson, M., Renstrom, L. H. M., Ottinger, T., Linne, T. (2006). Characterization of proteinase K-resistant N- and C-terminally truncated PrP in Nor98 atypical scrapie. J. Gen. Virol. 87: 1751-1760 [Abstract] [Full Text]  
  • Andrievskaia, O., McRae, H., Elmgren, C., Huang, H., Balachandran, A., Nielsen, K. (2006). Generation of Antibodies against Bovine Recombinant Prion Protein in Various Strains of Mice. CVI 13: 98-105 [Abstract] [Full Text]  
  • Rezaie, P., Pontikis, C. C., Hudson, L., Cairns, N. J., Lantos, P. L. (2005). Expression of Cellular Prion Protein in the Frontal and Occipital Lobe in Alzheimer's Disease, Diffuse Lewy Body Disease, and in Normal Brain: An Immunohistochemical Study. J. Histochem. Cytochem. 53: 929-940 [Abstract] [Full Text]  
  • Monleon, E., Monzon, M., Hortells, P., Vargas, A., Acin, C., Badiola, J. J. (2004). Detection of PrPsc on Lymphoid Tissues from Naturally Affected Scrapie Animals: Comparison of Three Visualization Systems. J. Histochem. Cytochem. 52: 145-151 [Abstract] [Full Text]  
  • Hamir, A. N., Miller, J. M., Cutlip, R. C. (2004). Failure to Detect Prion Protein (PrPres) by Immunohistochemistry in Striated Muscle Tissues of Animals Experimentally Inoculated with Agents of Transmissible Spongiform Encephalopathy. Vet Pathol 41: 78-81 [Abstract] [Full Text]  
  • Ueti, M. W., Palmer, G. H., Kappmeyer, L. S., Scoles, G. A., Knowles, D. P. (2003). Expression of Equi Merozoite Antigen 2 during Development of Babesia equi in the Midgut and Salivary Gland of the Vector Tick Boophilus microplus. J. Clin. Microbiol. 41: 5803-5809 [Abstract] [Full Text]  
  • Ersdal, C., Ulvund, M. J., Benestad, S. L., Tranulis, M. A. (2003). Accumulation of Pathogenic Prion Protein (PrPSc) in Nervous and Lymphoid Tissues of Sheep with Subclinical Scrapie. Vet Pathol 40: 164-174 [Abstract] [Full Text]  
  • Hamir, A. N., Miller, J. M., Cutlip, R. C., Stack, M. J., Chaplin, M. J., Jenny, A. L. (2003). Preliminary Observations on the Experimental Transmission of Scrapie to Elk (Cervus elaphus nelsoni) by Intracerebral Inoculation. Vet Pathol 40: 81-85 [Abstract] [Full Text]  
  • Sigurdson, C. J., Barillas-Mury, C., Miller, M. W., Oesch, B., van Keulen, L. J. M., Langeveld, J. P. M., Hoover, E. A. (2002). PrPCWD lymphoid cell targets in early and advanced chronic wasting disease of mule deer. J. Gen. Virol. 83: 2617-2628 [Abstract] [Full Text]  
  • O'Rourke, K. I., Duncan, J. V., Logan, J. R., Anderson, A. K., Norden, D. K., Williams, E. S., Combs, B. A., Stobart, R. H., Moss, G. E., Sutton, D. L. (2002). Active Surveillance for Scrapie by Third Eyelid Biopsy and Genetic Susceptibility Testing of Flocks of Sheep in Wyoming. CVI 9: 966-971 [Abstract] [Full Text]  
  • Spraker, T. R., Zink, R. R., Cummings, B. A., Sigurdson, C. J., Miller, M. W., O'Rourke, K. I. (2002). Distribution of Protease-resistant Prion Protein and Spongiform Encephalopathy in Free-ranging Mule Deer (Odocoileus hemionus) with Chronic Wasting Disease. Vet Pathol 39: 546-556 [Abstract] [Full Text]  
  • Tuo, W., O'Rourke, K. I., Zhuang, D., Cheevers, W. P., Spraker, T. R., Knowles, D. P. (2002). Pregnancy status and fetal prion genetics determine PrPSc accumulation in placentomes of scrapie-infected sheep. Proc. Natl. Acad. Sci. USA 99: 6310-6315 [Abstract] [Full Text]  
  • Spraker, T. R., Zink, R. R., Cummings, B. A., Wild, M. A., Miller, M. W., O'Rourke, K. I. (2002). Comparison of Histological Lesions and Immunohistochemical Staining of Proteinase-resistant Prion Protein in a Naturally Occurring Spongiform Encephalopathy of Free-ranging Mule Deer (Odocoileus hemionus) with Those of Chronic Wasting Disease of Captive Mule Deer. Vet Pathol 39: 110-119 [Abstract] [Full Text]  
  • O'Rourke, K. I., Baszler, T. V., Besser, T. E., Miller, J. M., Cutlip, R. C., Wells, G. A. H., Ryder, S. J., Parish, S. M., Hamir, A. N., Cockett, N. E., Jenny, A., Knowles, D. P. (2000). Preclinical Diagnosis of Scrapie by Immunohistochemistry of Third Eyelid Lymphoid Tissue. J. Clin. Microbiol. 38: 3254-3259 [Abstract] [Full Text]  
  • Sigurdson, C. J., Williams, E. S., Miller, M. W., Spraker, T. R., O'Rourke, K. I., Hoover, E. A. (1999). Oral transmission and early lymphoid tropism of chronic wasting disease PrPres in mule deer fawns (Odocoileus hemionus ). J. Gen. Virol. 80: 2757-2764 [Abstract] [Full Text]  
  • O'Rourke, K. I., Besser, T. E., Miller, M. W., Cline, T. F., Spraker, T. R., Jenny, A. L., Wild, M. A., Zebarth, G. L., Williams, E. S. (1999). PrP genotypes of captive and free-ranging Rocky Mountain elk (Cervus elaphus nelsoni) with chronic wasting disease. J. Gen. Virol. 80: 2765-2679 [Abstract] [Full Text]  
  • Tuo, W., Zhuang, D., Knowles, D. P., Cheevers, W. P., Sy, M.-S., O'Rourke, K. I. (2001). PrP-C and PrP-Sc at the Fetal-Maternal Interface. J. Biol. Chem. 276: 18229-18234 [Abstract] [Full Text]  
  • Tuo, W., O'Rourke, K. I., Zhuang, D., Cheevers, W. P., Spraker, T. R., Knowles, D. P. (2002). Pregnancy status and fetal prion genetics determine PrPSc accumulation in placentomes of scrapie-infected sheep. Proc. Natl. Acad. Sci. USA 99: 6310-6315 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»