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Journal of Clinical Microbiology, June 1998, p. 1769-1771, Vol. 36, No. 6
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Diagnosis of Group A Streptococcal Necrotizing Fasciitis by Using PCR To Amplify the Streptococcal Pyrogenic Exotoxin B Gene

Lisa Louie,1 Andrew E. Simor,1,2,* Marie Louie,1,2 Allison McGeer,2,3 and Donald E. Low2,3

Department of Microbiology, SD Laboratory Services, Sunnybrook Health Science Centre,1 Mount Sinai and Princess Margaret Hospitals,3 and the University of Toronto,2 Toronto, Ontario, Canada

Received 17 October 1997/Returned for modification 15 January 1998/Accepted 4 March 1998

This study evaluated a PCR assay for detection of the streptococcal pyrogenic exotoxin B (speB) gene from tissue biopsy specimens of patients with necrotizing fasciitis. speB was detected in specimens from all 10 patients with necrotizing fasciitis due to group A streptococcus. The assay was negative for all 11 patients without culture or serologic evidence of streptococcal infection. These results suggest that the detection of speB by PCR may be useful for confirming group A streptococcal infection when cultures are negative or not available.

* Corresponding author. Mailing address: Department of Microbiology, Sunnybrook Health Science Centre, B121-2075 Bayview Ave., North York, Ontario, Canada M4N 3M5. Phone: (416) 480-4549. Fax: (416) 480-6845. E-mail: andrew.simor{at}sunnybrook.on.ca.

Journal of Clinical Microbiology, June 1998, p. 1769-1771, Vol. 36, No. 6
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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