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Journal of Clinical Microbiology, June 1998, p. 1793-1794, Vol. 36, No. 6
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Reverse Transcriptase PCR Amplification of Rickettsia typhi from Infected Mammalian Cells and Insect Vectors

James A. Higgins,dagger Suzana Radulovic, Bruce H. Noden, Jill M. Troyer, and Abdu F. Azad*

Department of Microbiology and Immunology, University of Maryland Medical School, Baltimore, Maryland 21201

Received 14 October 1997/Returned for modification 15 January 1998/Accepted 19 March 1998

We developed a reverse transcriptase PCR assay to detect expression of 120- and 17-kDa antigen genes in Rickettsia typhi. Infected Vero cell and flea RNAs were reverse transcribed by using random hexamers. The cDNA was amplified by using high concentrations of primer and template in an inexpensive, nonradioactive assay.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of Maryland Medical School, 655 W. Baltimore St., Baltimore, MD 21201. Phone: (410) 706-3335. Fax: (410) 706-0282. E-mail: aazad{at}umaryland.edu.

dagger Present address: USDA Agricultural Research Service, LPSI, IDRL, Beltsville, MD 20705.

Journal of Clinical Microbiology, June 1998, p. 1793-1794, Vol. 36, No. 6
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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