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Journal of Clinical Microbiology, June 1998, p. 1798-1800, Vol. 36, No. 6
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Stabilized, Freeze-Dried PCR Mix for Detection of Mycobacteria

Paul R. Klatser,1,* Sjoukje Kuijper,1 Cor W. van Ingen,2 and Arend H. J. Kolk1

Department of Biomedical Research, Royal Tropical Institute, Amsterdam,1 and Foundation for the Advancement of Public Health and Environmental Protection (SVM), Bilthoven,2 The Netherlands

Received 12 November 1997/Returned for modification 7 January 1998/Accepted 24 February 1998

We report here the development of a freeze-drying procedure allowing stabilization at ambient temperature of preoptimized, premixed, and predispensed PCR mixes aimed at the detection of mycobacteria in clinical materials. The freeze-dried mixes retained activity at 4°C and at 20°C for 1 year and for 3 months at 37°C, as judged by their performance with 50 and 500 fg of purified Mycobacterium bovis BCG target DNA.

* Corresponding author. Mailing address: Department of Biomedical Research, Royal Tropical Institute, Meibergdreef 39, 1105 AZ Amsterdam, The Netherlands. Phone: 31-20-5665441. Fax: 31-20-6971841. E-mail: BO{at}mail.support.nl.

Journal of Clinical Microbiology, June 1998, p. 1798-1800, Vol. 36, No. 6
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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