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Journal of Clinical Microbiology, June 1998, p. 1801-1804, Vol. 36, No. 6
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
A PCR Specific for Escherichia coli O157
Based on the rfb Locus Encoding O157
Lipopolysaccharide
Patricia M.
Desmarchelier,1,*
Sima S.
Bilge,2
Narelle
Fegan,1
Leanne
Mills,1
James C.
Vary Jr.,2 and
Phillip I.
Tarr2
Commonwealth Scientific and Industrial
Research Organisation, Brisbane, Australia,1
and
Children's Hospital and Medical Center and the
University of Washington, Seattle, Washington2
Received 2 October 1997/Returned for modification 2 February
1998/Accepted 20 March 1998
A PCR was developed for the detection of Escherichia
coli O157 based on the rfbE O-antigen synthesis
genes. A 479-bp PCR product was amplified specifically from E. coli O157 in cell lysates containing 200 or 2 CFU
following crude DNA extraction. The PCR detected <1 CFU of
E. coli O157 per ml in raw milk following enrichment.
*
Corresponding author. Mailing address: CSIRO, P.O.
Box 3312, Tingalpa D.C., Queensland 4173, Australia. Phone: 61 7 3214 2000. Fax: 61 7 3214 2062. E-mail:
Patricia.Desmarchelier{at}foodscience.afisc.csiro.au.
Journal of Clinical Microbiology, June 1998, p. 1801-1804, Vol. 36, No. 6
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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