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Journal of Clinical Microbiology, June 1998, p. 1801-1804, Vol. 36, No. 6
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

A PCR Specific for Escherichia coli O157 Based on the rfb Locus Encoding O157 Lipopolysaccharide

Patricia M. Desmarchelier,1,* Sima S. Bilge,2 Narelle Fegan,1 Leanne Mills,1 James C. Vary Jr.,2 and Phillip I. Tarr2

Commonwealth Scientific and Industrial Research Organisation, Brisbane, Australia,1 and Children's Hospital and Medical Center and the University of Washington, Seattle, Washington2

Received 2 October 1997/Returned for modification 2 February 1998/Accepted 20 March 1998

A PCR was developed for the detection of Escherichia coli O157 based on the rfbE O-antigen synthesis genes. A 479-bp PCR product was amplified specifically from E. coli O157 in cell lysates containing 200 or 2 CFU following crude DNA extraction. The PCR detected <1 CFU of E. coli O157 per ml in raw milk following enrichment.

* Corresponding author. Mailing address: CSIRO, P.O. Box 3312, Tingalpa D.C., Queensland 4173, Australia. Phone: 61 7 3214 2000. Fax: 61 7 3214 2062. E-mail: Patricia.Desmarchelier{at}foodscience.afisc.csiro.au.

Journal of Clinical Microbiology, June 1998, p. 1801-1804, Vol. 36, No. 6
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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