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Journal of Clinical Microbiology, June 1998, p. 1801-1804, Vol. 36, No. 6
Commonwealth Scientific and Industrial
Research Organisation, Brisbane, Australia,1
and
Children's Hospital and Medical Center and the
University of Washington, Seattle, Washington2
Received 2 October 1997/Returned for modification 2 February
1998/Accepted 20 March 1998
A PCR was developed for the detection of Escherichia
coli O157 based on the rfbE O-antigen synthesis
genes. A 479-bp PCR product was amplified specifically from E. coli O157 in cell lysates containing 200 or 2 CFU
following crude DNA extraction. The PCR detected <1 CFU of
E. coli O157 per ml in raw milk following enrichment.
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
A PCR Specific for Escherichia coli O157
Based on the rfb Locus Encoding O157
Lipopolysaccharide
*
Corresponding author. Mailing address: CSIRO, P.O.
Box 3312, Tingalpa D.C., Queensland 4173, Australia. Phone: 61 7 3214 2000. Fax: 61 7 3214 2062. E-mail:
Patricia.Desmarchelier{at}foodscience.afisc.csiro.au.
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