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Journal of Clinical Microbiology, June 1998, p. 1814-1818, Vol. 36, No. 6
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Blinded, Externally Controlled Multicenter Evaluation of Light Microscopy and PCR for Detection of Microsporidia in Stool Specimens

Heinz Rinder,1,* Klaus Janitschke,2 Horst Aspöck,3 Alexandre J. Da Silva,4 Peter Deplazes,5 Daniel P. Fedorko,6 Caspar Franzen,7 Ursula Futh,8 Frank Hünger,9 Anselm Lehmacher,10 Christian G. Meyer,11 Jean-Michel Molina,12 Jörg Sandfort,13 Rainer Weber,14 Thomas Löscher,1 and the Diagnostic Multicenter Study Group on Microsporidiadagger

Department of Infectious Diseases and Tropical Medicine, University of Munich, Munich,1 Robert Koch Institute,2 Auguste Viktoria Hospital,8 Institute for Tropical Medicine, Humboldt University,11 and Virchow Clinic, Medical Polyclinic,13 Berlin, Clinic I for Internal Medicine, University of Cologne, Cologne,7 and Bernhard Nocht Institute,9 and Department of Bacteriology, Institute of Hygiene, Hamburg,10 Germany; Institute of Hygiene, University of Vienna, Vienna, Austria3; Centers for Disease Control and Prevention, Atlanta, Georgia 303414; National Institutes of Health, Bethesda, Maryland 208926; Institute of Parasitology, University of Zurich,5 and Division of Infectious Diseases and Hospital Epidemiology, University Hospital,14 Zurich, Switzerland; and Department of Infectious Diseases, Hospital Saint-Louis, University of Paris VII, Paris, France12

Received 19 December 1997/Returned for modification 13 February 1998/Accepted 20 March 1998

The quality parameters for the detection of microsporidia in identical sets of 50 stool samples were determined for six laboratories where technicians used light microscopy and for six laboratories where technicians used PCR. The average overall sensitivities were 67% (89% for patient samples only) for the PCR laboratories and 54% (80% for patient samples only) for the light microscopy laboratories. Specificities were 98 and 95%, respectively. Differences in results were most apparent between the individual laboratories rather than between the two major methods used.

* Corresponding author. Mailing address: Department of Infectious Diseases and Tropical Medicine, Leopoldstrasse 5, D-80802 Munich, Germany. Phone: 49-89-21803618. Fax: 49-89-336112. E-mail: rinder{at}lrz.uni-muenchen.de.

dagger Herbert Auer, Felicia David, Bernhard Fleischer, Andreas Haßl, Walter Heise, Thomas Hoppe, Olivier Liguory, Andreas Müller, Nancy A. Nelson, Otto Picher, Norman J. Pieniazek, and Angelika Thomschke.

Journal of Clinical Microbiology, June 1998, p. 1814-1818, Vol. 36, No. 6
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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