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Journal of Clinical Microbiology, July 1998, p. 1835-1839, Vol. 36, No. 7
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Monoclonal Antibody against Babesia equi: Characterization and Potential Application of Antigen for Serodiagnosis

Abgaandorjiin Avarzed,1 Ikuo Igarashi,1,* Daniel T. De Waal,2 Satoru Kawai,3 Yukio Oomori,4 Noboru Inoue,1 Yoshiyuki Maki,1 Yoshitaka Omata,5 Atsushi Saito,5 Hideyuki Nagasawa,1 Yutaka Toyoda,1 and Naoyoshi Suzuki1

The Research Center for Protozoan Molecular Immunology1 and Department of Veterinary Physiology,5 Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Department of Medical Zoology, Dokkyo University School of Medicine, Mibu, Tochigi 321-02,3 and Department of Anatomy, Asahikawa Medical College, Asahikawa, Hokkaido 070,4 Japan, and Parasitology Division, Onderstepoort Veterinary Institute, Onderstepoort 0110, South Africa2

Received 30 December 1997/Returned for modification 3 March 1998/Accepted 6 April 1998

Monoclonal antibody (MAb) BEG3 was produced against Babesia equi parasites to define a species-specific antigen for diagnostic use. The MAb reacted with single, paired, and Maltese cross forms of B. equi, and no reaction was observed with this MAb on acetone-fixed Babesia caballi, Babesia ovata, or Babesia microti parasites in the indirect immunofluorescent antibody test. Confocal laser and immunoelectron microscopic studies showed that the antigen which was recognized by this MAb was located on the surface of B. equi parasites. This MAb recognized a 19-kDa protein of B. equi antigen and did not react with B. caballi antigen or normal horse erythrocytes in immunoblot analysis. This MAb also significantly inhibited the in vitro growth of the B. equi parasite. Preliminary studies using partially purified antigen, which was separated by high-pressure liquid chromatography and recognized by the MAb, suggested that it is a suitable antigen for enzyme-linked immunosorbent assay detection of anti-B. equi antibodies in naturally infected horse sera.

* Corresponding author. Mailing address: The Research Center for Protozoan Molecular Immunology, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan. Phone: 81-155-49-5641. Fax: 81-155-49-5643. E-mail: igarcpmi{at}obihiro.ac.jp.

Journal of Clinical Microbiology, July 1998, p. 1835-1839, Vol. 36, No. 7
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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