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Journal of Clinical Microbiology, July 1998, p. 1882-1885, Vol. 36, No. 7
Laboratory of Microbiology,
Received 20 January 1998/Returned for modification 20 February
1998/Accepted 26 March 1998
To determine the types of Enterocytozoon bieneusi
strains associated with intestinal microsporidiosis, we developed a
rapid and efficient approach for typing parasites obtained from stool specimens by PCR-restriction fragment length polymorphism (PCR-RFLP). Typing was based on DNA polymorphism of the ribosomal DNA internal transcribed spacer (ITS) region of E. bieneusi. RFLPs
generated with two restriction enzymes (NlaIII and
Fnu4HI) in PCR-amplified ITS products were used to classify
strains into different lineages. This approach was successfully used to
differentiate 78 strains that had been obtained from the stools of 65 patients with intestinal microsporidiosis. Among the 78 strains, we
found four genetically unrelated lineages, showing the genetic
diversity of E. bieneusi. Type I strains of E. bieneusi were found in a majority of the samples, accounting for
51 (78%) of the 65 microsporidiosis cases. In contrast, type II, III,
and IV strains were found in only 8 (12%), 3 (5%), and 3 (5%) cases,
respectively. All strains of E. bieneusi we have tested so
far fall into one of four different lineages, and this study shows that
human intestinal microsporidiosis is most often associated with type I
strains. PCR-RFLP analysis of the ITS region of E. bieneusi
should be useful for epidemiological studies.
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Determination of Types of Enterocytozoon bieneusi
Strains Isolated from Patients with Intestinal
Microsporidiosis
*
Corresponding author. Mailing address: Clinique des
Maladies Infectieuses, Hôpital Saint-Louis, 1 avenue C. Vellefaux, 75475 Paris Cedex 10, France. Phone: (33) 1 42 49 90 66. Fax: (33) 1 42 49 90 67. E-mail: molina{at}chu-stlouis.fr.
Journal of Clinical Microbiology, July 1998, p. 1882-1885, Vol. 36, No. 7
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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