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Journal of Clinical Microbiology, July 1998, p. 1882-1885, Vol. 36, No. 7
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Determination of Types of Enterocytozoon bieneusi Strains Isolated from Patients with Intestinal Microsporidiosis

Olivier Liguory,1,2 Felicia David,2,3 Claudine Sarfati,4 Francis Derouin,4 and Jean-Michel Molina2,*

Laboratory of Microbiology, Hôpital Hôtel-Dieu,1 and Laboratory of Parasitology4 and Department of Infectious Diseases,2 Hôpital Saint-Louis, Paris, and Department of Internal Medicine, CHG Lagny Marne la Vallée, Lagny,3 France

Received 20 January 1998/Returned for modification 20 February 1998/Accepted 26 March 1998

To determine the types of Enterocytozoon bieneusi strains associated with intestinal microsporidiosis, we developed a rapid and efficient approach for typing parasites obtained from stool specimens by PCR-restriction fragment length polymorphism (PCR-RFLP). Typing was based on DNA polymorphism of the ribosomal DNA internal transcribed spacer (ITS) region of E. bieneusi. RFLPs generated with two restriction enzymes (NlaIII and Fnu4HI) in PCR-amplified ITS products were used to classify strains into different lineages. This approach was successfully used to differentiate 78 strains that had been obtained from the stools of 65 patients with intestinal microsporidiosis. Among the 78 strains, we found four genetically unrelated lineages, showing the genetic diversity of E. bieneusi. Type I strains of E. bieneusi were found in a majority of the samples, accounting for 51 (78%) of the 65 microsporidiosis cases. In contrast, type II, III, and IV strains were found in only 8 (12%), 3 (5%), and 3 (5%) cases, respectively. All strains of E. bieneusi we have tested so far fall into one of four different lineages, and this study shows that human intestinal microsporidiosis is most often associated with type I strains. PCR-RFLP analysis of the ITS region of E. bieneusi should be useful for epidemiological studies.

* Corresponding author. Mailing address: Clinique des Maladies Infectieuses, Hôpital Saint-Louis, 1 avenue C. Vellefaux, 75475 Paris Cedex 10, France. Phone: (33) 1 42 49 90 66. Fax: (33) 1 42 49 90 67. E-mail: molina{at}chu-stlouis.fr.

Journal of Clinical Microbiology, July 1998, p. 1882-1885, Vol. 36, No. 7
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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