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Journal of Clinical Microbiology, July 1998, p. 1948-1952, Vol. 36, No. 7
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Evaluation of the VITEK 2 System for Rapid Identification of Medically Relevant Gram-Negative Rods

Guido Funke,1,* Dominique Monnet,2,dagger Chiara deBernardis,1 Alexander von Graevenitz,1 and Jean Freney2

Department of Medical Microbiology, University of Zürich, CH-8028 Zürich, Switzerland,1 and UPRES EA 1655, Institut de Sciences Pharmaceutiques et Biologiques de Lyon, Faculté de Pharmacie Rockefeller, F-69373 Lyon Cedex 08, France2

Received 17 February 1998/Returned for modification 23 March 1998/Accepted 7 April 1998

The new VITEK 2 system (bioMérieux) was evaluated at two independent sites with the identification card for gram-negative bacilli (ID-GNB card). Of the 845 strains tested, which represented 70 different taxa belonging to either the family Enterobacteriaceae or the nonenteric bacilli, 716 (84.7%) were correctly identified at the species level. Thirty-two (3.8%) additional strains were identified to the species level after the performance of simple, rapid manual tests (oxidase, hemolysis, indole reaction, motility, and pigmentation). For 80 (9.5%) strains, these additional tests did not lead to an identification at the species level but the correct species identification was given among the organisms listed. Only 7 (0.8%) strains were misidentified, and 10 (1.2%) were not identified. Mistakes were randomly distributed over different taxa. Due to the new, more sensitive fluorescence-based technology of the VITEK 2 system, final results were available after 3 h. Since our evaluation was mainly a stress test, it is predicted that the VITEK 2 system in conjunction with the ID-GNB card would perform well under conditions of a routine clinical laboratory in identifying members of the family Enterobacteriaceae and selected species of nonenteric bacteria. This system is a promising, highly automated new tool for the rapid identification of gram-negative bacilli from human clinical specimens.

* Corresponding author. Mailing address: Department of Medical Microbiology, University of Zürich, Gloriastrasse 32, CH-8028 Zürich, Switzerland. Phone: +41-1-634-2701. Fax: +41-1-634-4906. E-mail: funke{at}immv.unizh.ch.

dagger Present address: Department of Gastrointestinal Infections, Statens Serum Institut, 2300 Copenhagen S, Denmark.

Journal of Clinical Microbiology, July 1998, p. 1948-1952, Vol. 36, No. 7
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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