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Journal of Clinical Microbiology, July 1998, p. 2043-2045, Vol. 36, No. 7
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Microtiter Assay for Detecting Campylobacter spp. and Helicobacter pylori with Surface Gangliosides Which Bind Cholera Toxin

David A. Sack,1,* Albert J. Lastovica,2 Sunny H. Chang,1 and Gary Pazzaglia3,4

Vaccine Testing Unit, Department of International Health, Johns Hopkins University, Baltimore, Maryland1; Department of Medical Microbiology, Red Cross War Memorial Children's Hospital, Cape Town, South Africa2; Enteric Diseases Program, Naval Medical Research Institute, Bethesda, Maryland3; and Department of Epidemiology, University of Texas School of Public Health, Houston, Texas4

Received 10 December 1997/Returned for modification 24 February 1998/Accepted 30 March 1998

Campylobacter jejuni with Gm1 ganglioside in the core of its lipopolysaccharide has been associated with Guillain-Barré syndrome. Since this epitope may be of considerable pathophysiologic importance and since this ganglioside binds cholera toxin, a rapid screening assay to detect bacteria that bind cholera toxin as an indication of Gm1 on their surfaces was developed. In the assay, bacterial lawns were grown on agar plates, harvested with phosphate-buffered saline, boiled, and incubated with a standard concentration of cholera B subunit. Preparations from strains with Gm1 were observed to inhibit the binding of cholera B subunit to Gm1 in a microtiter enzyme-linked immunosorbent assay. By using this assay with two groups of strains, 37 positive strains were detected among the 197 tested. Species with positive isolates included C. jejuni, Campylobacter coli, and Helicobacter pylori. The assay is capable of testing large numbers of isolates and should prove useful in future clinical and epidemiological studies of bacteria with this epitope.

* Corresponding author. Mailing address: Johns Hopkins University Vaccine Testing Unit, 550 N. Broadway, Suite 1001, Baltimore, MD 21205. Phone: (410) 955-0053. Fax: (410) 614-9483. E-mail: dsack{at}jhsph.edu.

Journal of Clinical Microbiology, July 1998, p. 2043-2045, Vol. 36, No. 7
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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