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Journal of Clinical Microbiology, July 1998, p. 2081-2083, Vol. 36, No. 7
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Molecular Diagnosis of Human Rhinovirus Infections: Comparison with Virus Isolation

Timo Hyypiä,1,* Tuomo Puhakka,2 Olli Ruuskanen,2 Mika Mäkelä,2 Anita Arola,1,2 and Pertti Arstila1

Departments of Virology1 and Pediatrics,2 University of Turku, FIN-20520 Turku, Finland

Received 31 December 1997/Returned for modification 9 March 1998/Accepted 3 April 1998

To compare the sensitivity and specificity of RT-PCR with that of virus isolation in the detection of human rhinoviruses, we tested nasopharyngeal aspirates from 200 patients on the 1st and 7th days after the onset of the common cold. An assay utilizing a short amplicon in the conserved 5' noncoding region was found highly sensitive. Of 192 positive samples altogether, 65 were found positive by RT-PCR only, 6 were positive by isolation exclusively, and 121 gave positive results in both tests.

* Corresponding author. Present address: Haartman Institute, Department of Virology, P.O. Box 21, FIN-00014 University of Helsinki, Helsinki, Finland. Phone: 358-9-1912 6466. Fax: 358-9-1912 6491. E-mail: Timo.Hyypia{at}helsinki.fi.

Journal of Clinical Microbiology, July 1998, p. 2081-2083, Vol. 36, No. 7
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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