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Journal of Clinical Microbiology, July 1998, p. 2096-2098, Vol. 36, No. 7
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Quantification of Human Immunodeficiency Virus Type 1 RNA Levels in Plasma by Using Small-Volume-Format Branched-DNA Assays

Torange Yeghiazarian,1,* Yuqi Zhao,2,3,4 Stanley E. Read,5 William Kabat,2 Xiaoyi Li,5 Sarah J. Hamren,1 Patrick J. Sheridan,1 Judith C. Wilber,1 David N. Chernoff,1 and Ram Yogev2,3

Chiron Diagnostics, Emeryville, California1; Section of Pediatric and Maternal HIV-1 Infections, Division of Infectious Diseases, Children's Memorial Hospital,2 and Department of Pediatrics3 and Department of Microbiology and Immunology,4 Northwestern University Medical School, Chicago, Illinois; and Division of Infectious Diseases, The Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada5

Received 5 December 1997/Returned for modification 11 February 1998/Accepted 3 April 1998

We have developed small-volume (50 or 250 µl)-format branched-DNA assays for human immunodeficiency virus type 1 (HIV-1) RNA for use with specimens in which the volume is limited and/or a high viral load is anticipated. These formats exhibited good correlation with the standard 1-ml format; high specificity, reproducibility, and linearity; and no significant difference in the quantification of HIV-1 subtypes.

* Corresponding author. Mailing address: Nucleic Acid Diagnostics and New Markers, Chiron Diagnostics, 4560 Horton Street, Emeryville, CA 94608-2916. Phone: (510) 923-3661. Fax: (510) 655-7733. E-mail: tory_yeghiazarian{at}cc.chiron.com.

Journal of Clinical Microbiology, July 1998, p. 2096-2098, Vol. 36, No. 7
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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