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Journal of Clinical Microbiology, July 1998, p. 2099-2102, Vol. 36, No. 7
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Clonal Nature of Enterotoxigenic Escherichia coli Serotype O6:H16 Revealed by Randomly Amplified Polymorphic DNA Analysis

Ana B. F. Pacheco,1,* Kelen C. Soares,1 Darcy F. de Almeida,1 Gloria I. Viboud,2 Norma Binsztein,2 and Luis C. S. Ferreira1

Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil,1 and Instituto Nacional de Enfermedades Infecciosas---ANLIS Carlos G. Malbrán, Buenos Aires,2 Argentina

Received 7 October 1997/Returned for modification 17 February 1998/Accepted 6 April 1998

The genetic relatedness among 29 enterotoxigenic Escherichia coli strains of serotype O6:H16 was investigated by randomly amplified polymorphic DNA (RAPD) analysis. The strains were isolated in different parts of the world, displayed CS1-CS3 or CS2-CS3 profiles, and expressed heat-labile toxin (LT) and heat-stable toxin; a single strain expressed only LT. Ten RAPD types were distinguished and showed significant similarity, having on average 82% of the amplified bands in common. These results indicated that, irrespective of the different geographical origin or virulence factors, these strains belonged to a widespread clonal group.

* Corresponding author. Mailing address: Lab. Fisiol. Cel., Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, CCS, Cidade Universitária, Rio de Janeiro RJ 21941-590, Brazil. Phone: 55 21 5601484. Fax: 55 21 2808193. E-mail: biafp{at}ibccf.biof.ufrj.br.

Journal of Clinical Microbiology, July 1998, p. 2099-2102, Vol. 36, No. 7
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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