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Journal of Clinical Microbiology, July 1998, p. 2112-2114, Vol. 36, No. 7
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Use of Fluorescent-Antibody Staining of Cytocentrifuge-Prepared Smears in Combination with Cell Culture for Direct Detection of Respiratory Viruses

Kirk M. Doing,1,2,* Mary Ann Jerkofsky,1 Elaine G. Dow,2 and Jo Ann Jellison2

Department of Microbiology, Biochemistry and Molecular Biology, University of Maine, Orono, Maine 04469,1 and Affiliated Laboratory, Inc., Bangor, Maine 044012

Received 9 December 1997/Returned for modification 2 February 1998/Accepted 30 March 1998

Over a 3-year period, 1,003 respiratory samples were collected and examined for selected respiratory viruses with cytocentrifuged prepared smears stained with fluorescently labeled antibodies (IFA) in conjunction with cell culture. IFA results were compared with results obtained by cell culture. Viruses were isolated or detected by direct means in 401 samples. Agreement between culture and IFA was 90%.

* Corresponding author. Mailing address: Affiliated Laboratory, Inc., 925 Union Street, Suite 4, Bangor, ME 04401. Phone: (207) 973-6900. Fax: (207) 947-2838. E-mail: doing{at}maine.maine.edu.

Journal of Clinical Microbiology, July 1998, p. 2112-2114, Vol. 36, No. 7
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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