Isolation and Characterization of Sorbitol-Fermenting Shiga Toxin (Verocytotoxin)-Producing Escherichia coli O157:Hsstrains in the Czech Republic

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Journal of Clinical Microbiology, July 1998, p. 2135-2137, Vol. 36, No. 7
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Isolation and Characterization of Sorbitol-Fermenting Shiga Toxin (Verocytotoxin)-Producing Escherichia coli O157:Hßtrains in the Czech Republic

Martina Bielaszewska,¹^,^* Herbert Schmidt,² Mohamed A. Karmali,³ Rasik Khakhria,⁴ Jan Janda,⁵ Kv $e$ ta Bláhová,⁵ and Helge Karch²

Institute of Medical Microbiology, The 2nd Medical Faculty, Charles University,¹ and 1st Clinic of Pediatrics, University Hospital Motol,⁵ 150 06 Prague, Czech Republic; Institut für Hygiene und Mikrobiologie der Universität Würzburg, 97080 Würzburg, Germany²; and Division of Microbiology, The Hospital for Sick Children, Toronto, Ontario M5G 1X8,³ and National Laboratory for Enteric Pathogens, Laboratory Centre for Disease Control, Ottawa, Ontario K1A 0L2,⁴ Canada

Received 16 December 1997/Returned for modification 9 March 1998/Accepted 13 April 1998

Two sorbitol-fermenting (SF) Shiga toxin-producing Escherichia coli (STEC) O157:H $-$ strains were isolated from patients withhemolytic-uremic syndrome in the Czech Republic in 1995. Theirphenotypic and genotypic characteristics and genomic DNA fingerprintswere identical or closely related to those of SF STEC O157:H $-$ strains isolated in Germany in 1988 to 1997. This indicates thatthe Czech isolates belong to the SF STEC O157 clone which is widespreadin Germany. It is the first finding of the clone outside Germany.

^* Corresponding author. Mailing address: Institute of Medical Microbiology, The 2nd Medical Faculty, Charles University, V úvalu84, 150 06 Prague 5-Motol, Czech Republic. Phone: 420-2-2443-5351.Fax: 420-2-2443-2020. E-mail: jan.janda{at}lfmotol.cuni.c2.

Journal of Clinical Microbiology, July 1998, p. 2135-2137, Vol. 36, No. 7
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

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