This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Descamps, D.
Right arrow Articles by Brun-Vézinet, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Descamps, D.
Right arrow Articles by Brun-Vézinet, F.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, July 1998, p. 2143-2145, Vol. 36, No. 7
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Line Probe Assay for Detection of Human Immunodeficiency Virus Type 1 Mutations Conferring Resistance to Nucleoside Inhibitors of Reverse Transcriptase: Comparison with Sequence Analysis

Diane Descamps,1,* Vincent Calvez,2 Gilles Collin,1 Agnès Cécille,2 Cristian Apetrei,1 Florence Damond,1 Christine Katlama,3 Sophie Matheron,4 Jean-Marie Huraux,2 and Françoise Brun-Vézinet1

Laboratoire de Virologie1 and Service des Malades Infectieuses et Tropicales,4 Hôpital Bichat-Claude Bernard, and Laboratoire de Virologie2 and Service des Maladies Infectieuses et Tropicales,3 Hôpital Pitié-Salpétriêre, Paris, France

Received 25 February 1998/Returned for modification 31 March 1998/Accepted 14 April 1998

We compared the line probe assay (LiPA) to sequence analysis for the detection of mutations conferring resistance to nucleoside inhibitors of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT). Plasma samples from 40 patients who had received zidovudine, dideoxyinosine, and dideoxycytosine, alone or in combination, and who were enrolled in the ALTIS 2 clinical trial (lamivudine [3TC] plus stavudine) were tested at enrollment and at week 24. RT PCR products from plasma were used for LiPA, and DNA was used for sequence analysis. LiPA gave uninterpretable results for 8.5% of the analyzed codons corresponding to 63 samples, mainly for codons 41, 69, and 70. Several minor discrepancies between the two methods occurred, mainly due to the ability of LiPA to detect mixed populations while sequence analyses detect a single homogeneous population. LiPA is suitable for detecting mixed populations and easy to implement in clinical laboratories and might be useful for epidemiological surveys of primary HIV-1 resistance.

* Corresponding author. Mailing address: Laboratoire de Virologie, Hôpital Bichat-Claude Bernard, 46 Rue Henri Huchard, 75018 Paris, France. Phone: 33-1.40.25.88.96. Fax: 33-1.46.27.02.08. E-mail: diane.descamps{at}bch.ap-hop-paris.fr.

Journal of Clinical Microbiology, July 1998, p. 2143-2145, Vol. 36, No. 7
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Petrella, M., Oliveira, M., Moisi, D., Detorio, M., Brenner, B. G., Wainberg, M. A. (2004). Differential Maintenance of the M184V Substitution in the Reverse Transcriptase of Human Immunodeficiency Virus Type 1 by Various Nucleoside Antiretroviral Agents in Tissue Culture. Antimicrob. Agents Chemother. 48: 4189-4194 [Abstract] [Full Text]  
  • Servais, J., Lambert, C., Fontaine, E., Plesséria, J.-M., Robert, I., Arendt, V., Staub, T., Schneider, F., Hemmer, R., Burtonboy, G., Schmit, J.-C. (2001). Comparison of DNA Sequencing and a Line Probe Assay for Detection of Human Immunodeficiency Virus Type 1 Drug Resistance Mutations in Patients Failing Highly Active Antiretroviral Therapy. J. Clin. Microbiol. 39: 454-459 [Abstract] [Full Text]  
  • Samri, A., Haas, G., Duntze, J., Bouley, J.-M., Calvez, V., Katlama, C., Autran, B. (2000). Immunogenicity of Mutations Induced by Nucleoside Reverse Transcriptase Inhibitors for Human Immunodeficiency Virus Type 1-Specific Cytotoxic T Cells. J. Virol. 74: 9306-9312 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»