This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Goh, S. H.
Right arrow Articles by Smith, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Goh, S. H.
Right arrow Articles by Smith, J. A.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, July 1998, p. 2164-2166, Vol. 36, No. 7
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Streptococcus iniae, a Human and Animal Pathogen: Specific Identification by the Chaperonin 60 Gene Identification Method

Swee Han Goh,1,2,* David Driedger,2 Sandra Gillett,2 Donald E. Low,3 Sean M. Hemmingsen,4 Mayben Amos,2 David Chan,2 Marguerite Lovgren,5 Barbara M. Willey,3 Carol Shaw,2 and John A. Smith1,2

Department of Pathology and Laboratory Medicine, University of British Columbia,1 and the Provincial Laboratory, British Columbia Centre for Disease Control Society,2 Vancouver, British Columbia, University of Toronto, Toronto, Ontario,3 National Research Council Canada, Plant Biotechnology Institute, Saskatoon, Saskatchewan,4 and National Centre for Streptococcus, Edmonton, Alberta,5 Canada

Received 26 November 1997/Returned for modification 13 March 1998/Accepted 23 April 1998

It was recently reported that Streptococcus iniae, a bacterial pathogen of aquatic animals, can cause serious disease in humans. Using the chaperonin 60 (Cpn60) gene identification method with reverse checkerboard hybridization and chemiluminescent detection, we identified correctly each of 12 S. iniae samples among 34 aerobic gram-positive isolates from animal and clinical human sources.


* Corresponding author. Mailing address: Department of Pathology and Laboratory Medicine, University of British Columbia and the Provincial Laboratory, BCCDC Society, 655W 12th Ave., Vancouver, British Columbia, Canada. V5Z 4R4. Phone: (604) 660-6005. Fax: (604) 660-0403. E-mail: shgoh{at}unixg.ubc.ca.


Journal of Clinical Microbiology, July 1998, p. 2164-2166, Vol. 36, No. 7
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.



This article has been cited by other articles:

  • Nawawi, R. A., Baiano, J. C. F., Kvennefors, E. C. E., Barnes, A. C. (2009). Host-Directed Evolution of a Novel Lactate Oxidase in Streptococcus iniae Isolates from Barramundi (Lates calcarifer). Appl. Environ. Microbiol. 75: 2908-2919 [Abstract] [Full Text]  
  • Haigh, J. C., Gerwing, V., Erdenebaatar, J., Hill, J. E. (2008). A NOVEL CLINICAL SYNDROME AND DETECTION OF ANAPLASMA OVIS IN MONGOLIAN REINDEER (RANGIFER TARANDUS). J Wildl Dis 44: 569-577 [Abstract] [Full Text]  
  • Chen, H.-J., Tsai, J.-C., Chang, T.-C., Hung, W.-C., Tseng, S.-P., Hsueh, P.-R., Teng, L.-J. (2008). PCR-RFLP assay for species and subspecies differentiation of the Streptococcus bovis group based on groESL sequences. J Med Microbiol 57: 432-438 [Abstract] [Full Text]  
  • Hill, J. E., Paccagnella, A., Law, K., Melito, P. L., Woodward, D. L., Price, L., Leung, A. H., Ng, L.-K., Hemmingsen, S. M., Goh, S. H. (2006). Identification of Campylobacter spp. and discrimination from Helicobacter and Arcobacter spp. by direct sequencing of PCR-amplified cpn60 sequences and comparison to cpnDB, a chaperonin reference sequence database.. J Med Microbiol 55: 393-399 [Abstract] [Full Text]  
  • Hung, W.-C., Tsai, J.-C., Hsueh, P.-R., Chia, J.-S., Teng, L.-J. (2005). Species identification of mutans streptococci by groESL gene sequence. J Med Microbiol 54: 857-862 [Abstract] [Full Text]  
  • Hill, J. E., Hemmingsen, S. M., Goldade, B. G., Dumonceaux, T. J., Klassen, J., Zijlstra, R. T., Goh, S. H., Van Kessel, A. G. (2005). Comparison of Ileum Microflora of Pigs Fed Corn-, Wheat-, or Barley-Based Diets by Chaperonin-60 Sequencing and Quantitative PCR. Appl. Environ. Microbiol. 71: 867-875 [Abstract] [Full Text]  
  • Tsai, J.-C., Hsueh, P.-R., Lin, H.-M., Chang, H.-J., Ho, S.-W., Teng, L.-J. (2005). Identification of Clinically Relevant Enterococcus Species by Direct Sequencing of groES and Spacer Region. J. Clin. Microbiol. 43: 235-241 [Abstract] [Full Text]  
  • Hill, J. E., Penny, S. L., Crowell, K. G., Goh, S. H., Hemmingsen, S. M. (2004). cpnDB: A Chaperonin Sequence Database. Genome Res 14: 1669-1675 [Abstract] [Full Text]  
  • Lee, J.-H., Park, H.-S., Jang, W.-J., Koh, S.-E., Kim, J.-M., Shim, S.-K., Park, M.-Y., Kim, Y.-W., Kim, B.-J., Kook, Y.-H., Park, K.-H., Lee, S.-H. (2003). Differentiation of Rickettsiae by groEL Gene Analysis. J. Clin. Microbiol. 41: 2952-2960 [Abstract] [Full Text]  
  • Lau, S. K. P., Woo, P. C. Y., Tse, H., Leung, K.-W., Wong, S. S. Y., Yuen, K.-Y. (2003). Invasive Streptococcus iniae Infections Outside North America. J. Clin. Microbiol. 41: 1004-1009 [Abstract] [Full Text]  
  • Kwok, A. Y. C., Chow, A. W. (2003). Phylogenetic study of Staphylococcus and Macrococcus species based on partial hsp60 gene sequences. Int. J. Syst. Evol. Microbiol. 53: 87-92 [Abstract] [Full Text]  
  • Teng, L.-J., Hsueh, P.-R., Tsai, J.-C., Chen, P.-W., Hsu, J.-C., Lai, H.-C., Lee, C.-N., Ho, S.-W. (2002). groESL Sequence Determination, Phylogenetic Analysis, and Species Differentiation for Viridans Group Streptococci. J. Clin. Microbiol. 40: 3172-3178 [Abstract] [Full Text]  
  • Hill, J. E., Seipp, R. P., Betts, M., Hawkins, L., Van Kessel, A. G., Crosby, W. L., Hemmingsen, S. M. (2002). Extensive Profiling of a Complex Microbial Community by High-Throughput Sequencing. Appl. Environ. Microbiol. 68: 3055-3066 [Abstract] [Full Text]  
  • Brousseau, R., Hill, J. E., Prefontaine, G., Goh, S.-H., Harel, J., Hemmingsen, S. M. (2001). Streptococcus suis Serotypes Characterized by Analysis of Chaperonin 60 Gene Sequences. Appl. Environ. Microbiol. 67: 4828-4833 [Abstract] [Full Text]  
  • Teng, L.-J., Hsueh, P.-R., Wang, Y.-H., Lin, H.-M., Luh, K.-T., Ho, S.-W. (2001). Determination of Enterococcus faecalis groESL Full-Length Sequence and Application for Species Identification. J. Clin. Microbiol. 39: 3326-3331 [Abstract] [Full Text]  
  • Goh, S. H., Facklam, R. R., Chang, M., Hill, J. E., Tyrrell, G. J., Burns, E. C. M., Chan, D., He, C., Rahim, T., Shaw, C., Hemmingsen, S. M. (2000). Identification of Enterococcus Species and Phenotypically Similar Lactococcus and Vagococcus Species by Reverse Checkerboard Hybridization to Chaperonin 60 Gene Sequences. J. Clin. Microbiol. 38: 3953-3959 [Abstract] [Full Text]  
  • Kawamura, Y., Whiley, R. A., Shu, S.-E., Ezaki, T., Hardie, J. M. (1999). Genetic approaches to the identification of the mitis group within the genus Streptococcus. Microbiology 145: 2605-2613 [Abstract] [Full Text]