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Journal of Clinical Microbiology, August 1998, p. 2200-2204, Vol. 36, No. 8
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Genetic Relationship between Cryptococcus neoformans var. neoformans Strains of Serotypes A and D

Sarah P. Franzot,dagger Bettina C. Fries, Wendy Cleare, and Arturo Casadevall*

Department of Medicine and Department of Microbiology and Immunology, Division of Infectious Diseases, Albert Einstein College of Medicine, Bronx, New York

Received 24 February 1998/Returned for modification 17 April 1998/Accepted 1 May 1998

Cryptococcus neoformans serotypes A and D are responsible for the overwhelming majority of infections in patients with AIDS. The genetic relationship between the serotypes is poorly understood, but there are significant differences in the epidemiology and clinical presentation of serotype A and D infections. We evaluated the genetic relationship between reference C. neoformans strains belonging to serotypes A and D by analyzing their URA5 sequences and restriction fragment length polymorphisms (RFLPs) with the C. neoformans repetitive element 1 (CNRE-1) probe. The results were compared to those previously obtained for isolates from Brazil and New York City by the same typing methods, and dendrograms were generated. Serotype A and D strains produced distinct RFLP patterns consistent with their separation into two major clusters in the dendrogram generated on the basis of RFLP data. Similarly, serotype A and D strains clustered independently on the basis of the nucleotide sequences of their URA5 genes. Pairwise comparisons revealed average numbers of nucleotide differences within serotypes A and D of 3.0 ± 1.7 and 7.2 ± 3.4, respectively (P < 0.0001), and between serotypes A and D of 41.9 ± 2.7. In summary, our results indicate phylogenetic differences between the two serotypes of C. neoformans var. neoformans and suggest that these serotypes could probably be considered different varieties of C. neoformans.

* Corresponding author. Mailing address: Albert Einstein College of Medicine, 1300 Morris Park Ave., Golding Building, Room 701, Bronx, NY 10461. Phone: (718) 430-4259. Fax: (718) 430-8701. E-mail: casadeva{at}aecom.yu.edu.

dagger Present address: Department of Pharmacology, Cornell University Medical College, New York, NY 10021.

Journal of Clinical Microbiology, August 1998, p. 2200-2204, Vol. 36, No. 8
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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