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Journal of Clinical Microbiology, August 1998, p. 2254-2257, Vol. 36, No. 8
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Evaluation of Mannitol Salt Agar for Detection of Oxacillin Resistance in Staphylococcus aureus by Disk Diffusion and Agar Screening

Günter Kampf,* Christoph Lecke, Ann-Katrin Cimbal, Klaus Weist, and Henning Rüden

Institut für Hygiene, Freie Universität Berlin, Berlin, Germany

Received 16 January 1998/Returned for modification 5 March 1998/Accepted 24 April 1998

Mannitol salt agar was evaluated for detection of oxacillin resistance in 136 Staphylococcus aureus isolates. All mecA-positive isolates (n = 54) were correctly categorized as oxacillin resistant by the disk diffusion test (1-µg disk; zone diameter, <16 mm); the specificity was 97.6%. Agar screening (2 µg of oxacillin per ml) revealed a sensitivity of 98.1% and a specificity of 95.1%.

* Corresponding author. Mailing address: Institut für Hygiene, Freie Universität Berlin, Hindenburgdamm 27, 12203 Berlin, Germany. Phone: 49 30 8445 3680. Fax: 49 30 8445 3682.

Journal of Clinical Microbiology, August 1998, p. 2254-2257, Vol. 36, No. 8
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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