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Journal of Clinical Microbiology, August 1998, p. 2314-2321, Vol. 36, No. 8
Laboratory of Enteric Pathogens, Central
Public Health Laboratory, London NW9 5HT, United Kingdom
Received 17 February 1998/Returned for modification 15 April
1998/Accepted 15 May 1998
Pulsed-field gel electrophoresis (PFGE) was used to resolve
XbaI and SpeI macrorestriction fragments from
60 defined phage type (PT) reference strains of Salmonella
enteritidis. The level of discrimination was compared to that
afforded by plasmid profile analysis and ribotyping. Twenty-eight
distinct XbaI pulsed-field profiles (PFPs) were observed,
although a single type, PFP X1, predominated. Absence of the 57-kb
spv-associated fragment was observed for three PT reference
strains, and the profile was designated PFP X1A. The XbaI
macrorestriction profiles of a further four PT reference strains were
altered by the presence of plasmid-associated bands. Twenty-six
SpeI-generated PFPs (plus one subtype) were observed for
the same strains. No SpeI fragment corresponding to the
38-MDa serovar-specific plasmid was detected. The distribution of
XbaI and SpeI profiles did not always
correspond, producing a total of 32 combined PFPs for the 60 PT
reference strains. This compared with a total of 18 different plasmid
profiles and three PvuII ribotypes generated by the same
strains. The results of this study indicate that PFGE may offer an
improved level of discrimination over other genotypic typing methods
for the epidemiological typing of S. enteritidis.
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Genotypic Characterization of Salmonella
enteritidis Phage Types by Plasmid Analysis, Ribotyping,
and Pulsed-Field Gel Electrophoresis
*
Corresponding author. Mailing address: Laboratory of
Enteric Pathogens, Central Public Health Laboratory, 61 Colindale Ave., London NW9 5HT, United Kingdom. Phone: 0181 200 4400. Fax: 0181 905 9929. E-mail: jthrelfall{at}phls.co.uk.
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