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Journal of Clinical Microbiology, August 1998, p. 2322-2325, Vol. 36, No. 8
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Isolation of Porphyromonas gingivalis and Detection of Immunoglobulin A Specific to Fimbrial Antigen in Gingival Crevicular Fluid

Francesca Condorelli,1,* Guido Scalia,1 Giuditta Calì,2 Bruno Rossetti,2 Giuseppe Nicoletti,1 and Anna M. Lo Bue1

Institute of Microbiology1 and Clinic of Dentistry, Section of Parodontology,2 University of Catania, Catania, Italy

Received 6 October 1997/Returned for modification 8 December 1997/Accepted 13 May 1998

The present study evaluated the prevalence of Porphyromonas gingivalis and the correlation between the bacterial culture method and the detection of immunoglobulin A (IgA) specific to the P. gingivalis fimbrial antigen in gingival crevicular fluid (GCF). P. gingivalis was isolated from 78.3% of subgingival plaque samples obtained from active sites and 34.7% of those from inactive sites of periodontal patients. P. gingivalis was isolated from only 4.7% of healthy subjects (control group). Immunoglobulins specific to the P. gingivalis fimbrial antigen were detected by enzyme-linked immunosorbent assay (ELISA). The overall agreement between the results of the P. gingivalis culture method and the results of specific IgA detection in periodontal patients was 71.7% for active sites and 58.7% for inactive sites. IgA specific to P. gingivalis was absent in GCF from all of the sites of healthy subjects. The results suggest that P. gingivalis is associated with the local production of specific IgA. The detection of IgA antibodies specific to P. gingivalis in GCF by ELISA may be used as a predictive parameter to reveal the early phase of the activation of recurrent periodontal infections.

* Corresponding author. Mailing address: Institute of Microbiology, University of Catania, Via Androne, 81, 95124 Catania, Italy. Phone: 0039-(0)95-312651. Fax: 0039-(0)95-316214. E-mail: gscalia{at}dimtel.nti.it.

Journal of Clinical Microbiology, August 1998, p. 2322-2325, Vol. 36, No. 8
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Kobayashi, T., Yamamoto, K., Sugita, N., van Spriel, A. B., Kaneko, S., van de Winkel, J. G. J., Yoshie, H. (2001). Effective In Vitro Clearance of Porphyromonas gingivalis by Fc{alpha} Receptor I (CD89) on Gingival Crevicular Neutrophils. Infect. Immun. 69: 2935-2942 [Abstract] [Full Text]  
  • Podmore, M., Ebersole, J.L., Kinane, D.F. (2001). Immunodominant Antigens in Periodontal Disease: a Real or Illusive Concept?. CROBM 12: 179-185 [Abstract] [Full Text]  


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