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Journal of Clinical Microbiology, August 1998, p. 2333-2335, Vol. 36, No. 8
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Screening of Stool Samples for Identification of Vancomycin-Resistant Enterococcus Isolates Should Include the Methyl-alpha -DGlucopyranoside Test To Differentiate Nonmotile Enterococcus gallinarum from E. faecium

Christine Y. Turenne,1,2,* Daryl J. Hoban,1,2 James A. Karlowsky,1,2,3 George G. Zhanel,1,2,3,4 and Amin M. Kabani1,2,4

Department of Medical Microbiology, Faculty of Medicine,1 and Faculty of Pharmacy,3 University of Manitoba, and Departments of Clinical Microbiology2 and Medicine,4 Health Sciences Centre, Winnipeg, Manitoba R3A 1R9, Canada

Received 6 February 1998/Returned for modification 24 March 1998/Accepted 12 May 1998

The methyl-alpha -D-glucopyranoside (MDG) test has been shown to be superior to motility testing in differentiating Enterococcus faecium from E. gallinarum. In the present study, 33 vancomycin-resistant enterococcus (VRE) isolates collected as part of a stool surveillance study were compared by using motility and MDG. Motility testing identified all 33 isolates as E. faecium, whereas MDG identified 11 of the 33 isolates as nonmotile E. gallinarum. The MDG results were confirmed by sequencing the 16S rDNA V6-to-V8 region. We conclude that the MDG test is a necessary component of routine VRE screening.

* Corresponding author. Mailing address: Department of Clinical Microbiology, Health Sciences Centre, MS6-820 Sherbrook St., Winnipeg, Manitoba R3A 1R9, Canada. Phone: (204) 787-4696. Fax: (204) 787-4699. E-mail: umturen0{at}cc.UManitoba.ca.

Journal of Clinical Microbiology, August 1998, p. 2333-2335, Vol. 36, No. 8
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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