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Journal of Clinical Microbiology, August 1998, p. 2336-2338, Vol. 36, No. 8
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Localization of Persistent Enterocytozoon bieneusi Infection in Normal Rhesus Macaques (Macaca mulatta) to the Hepatobiliary Tree

Keith G. Mansfield,1,* Angela Carville,1,2 Daniel Hebert,1 Laura Chalifoux,1 Daniel Shvetz,1 K. C. Lin,1 S. Tzipori,2 and Andrew A. Lackner1

New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts 01772,1 and Division of Infectious Diseases, Tufts University School of Veterinary Medicine, North Grafton, Massachusetts 015362

Received 2 February 1998/Returned for modification 23 April 1998/Accepted 11 May 1998

Enterocytozoon bieneusi is the most common microsporidian parasite recognized in human patients with AIDS. Recently, we identified a virtually identical organism causing a spontaneous infection associated with hepatobiliary and intestinal disease in simian immunodeficiency virus (SIV)-infected macaques. To examine the natural history of the infection, we examined captive rhesus macaques for E. bieneusi by PCR, in situ hybridization, and cytochemical techniques. PCR performed on fecal DNA detected enterocytozoon infection in 22 (16.7%) of 131 normal rhesus macaques (Macaca mulatta), compared to 18 (33.8%) of 53 rhesus macaques experimentally inoculated with SIV. In normal rhesus macaques, persistence of infection was demonstrated for up to 262 days and was usually not associated with clinical signs. In six of seven normal rhesus animals, E. bieneusi was detected by PCR in bile obtained through percutaneous cholecystocentesis but not by in situ hybridization performed on endoscopic biopsies of duodenum and proximal jejunum.

* Corresponding author. Mailing address: Harvard Medical School, New England Regional Primate Research Center, P.O. Box 9102, Southborough, MA 01772-9012. Phone: (508) 624-8183. Fax: (508) 624-8181. E-mail: kmansfie{at}warren.med.harvard.edu.

Journal of Clinical Microbiology, August 1998, p. 2336-2338, Vol. 36, No. 8
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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