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Journal of Clinical Microbiology, August 1998, p. 2349-2352, Vol. 36, No. 8
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Efficient Isolation of Human Immunodeficiency Virus Type 1 RNA from Cervical Swabs

Adeline M. Hajjar,1,2 Paul F. Lewis,1,3,dagger Yohannes Endeshaw,4,5 Jackoniah Ndinya-Achola,5 Joan K. Kreiss,4,6 and Julie Overbaugh1,*

Departments of Microbiology,1 Comparative Medicine,2 Pediatrics,3 Epidemiology,4 and Medicine,6 University of Washington, Seattle, Washington 98195, and Department of Medical Microbiology, University of Nairobi, Kenya5

Received 30 December 1997/Returned for modification 27 April 1998/Accepted 13 May 1998

An efficient method for the isolation of human immunodeficiency virus type 1 (HIV-1) nucleic acids from dry cervical swabs was developed. HIV-1 gag and env were detected in 96% (25 of 26) and 81% (21 of 26), respectively, of the samples tested by PCR from HIV-1-seropositive women in a Kenyan cohort study. Eighty-eight percent of the swabs (22 of 25) were positive for gag RNA, and 85% (17 of 20) were positive for env RNA. Fewer than 1,000 copies of HIV-1 gag RNA were detected in four swabs in which a competitive quantitative PCR assay was used. The method described here may be useful for both qualitative and quantitative analyses of HIV RNA in mucosal secretions as well as amplification and cloning of full-length viral genes for functional studies.

* Corresponding author. Mailing address: Dept. of Microbiology, University of Washington, Seattle, WA 98195. Phone: (206) 543-3146. Fax: (206) 543-8297. E-mail: overbaug{at}u.washington.edu.

dagger Present address: Department of Pediatrics, Oregon Health Sciences University, Portland, OR 97201.

Journal of Clinical Microbiology, August 1998, p. 2349-2352, Vol. 36, No. 8
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Sagar, M., Kirkegaard, E., Long, E. M., Celum, C., Buchbinder, S., Daar, E. S., Overbaugh, J. (2004). Human Immunodeficiency Virus Type 1 (HIV-1) Diversity at Time of Infection Is Not Restricted to Certain Risk Groups or Specific HIV-1 Subtypes. J. Virol. 78: 7279-7283 [Abstract] [Full Text]  


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