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Journal of Clinical Microbiology, August 1998, p. 2359-2362, Vol. 36, No. 8
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Rapid Screening for Penicillin Susceptibility of Systemic Pneumococcal Isolates by Restriction Enzyme Profiling of the pbp2B Gene

Bernard Beall,* Richard R. Facklam, Delois M. Jackson, and Holly H. Starling

Centers for Disease Control and Prevention, Childhood and Respiratory Infections, Atlanta, Georgia 30333

Received 23 March 1998/Returned for modification 30 April 1998/Accepted 13 May 1998

Restriction digest profiling of pneumococcal pbp2b-specific amplicons was effective for screening penicillin resistance. The pbp2b amplicon of all pneumococcal isolates for which the MICs of penicillin were <= 0.03 µg/ml had one of two different susceptible restriction profiles, and all 33 isolates for which MICs were 0.5 µg/ml or greater had one of seven distinct resistant profiles. Low-concentration penicillin resistance (MICs = 0.06 µg/ml to 0.25 µg/ml) was associated with sensitive HaeIII profiles in some isolates; however, RsaI profiling and pbp2b sequence analysis of such isolates revealed that some isolates contained low-level resistant pbp2b alleles, while others had susceptible pbp2b alleles. This data indicates that low-level penicillin resistance is sometimes conferred by determinants other than pbp2b.

* Corresponding author. Mailing address: Centers for Disease Control and Prevention, Childhood and Respiratory Infections, 1600 Clifton Rd., Mailstop C02, Atlanta, GA 30333. Phone: (404) 639-1237. Fax: (404) 639-3123. E-mail: beb0{at}cdc.gov.

Journal of Clinical Microbiology, August 1998, p. 2359-2362, Vol. 36, No. 8
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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