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Journal of Clinical Microbiology, August 1998, p. 2366-2368, Vol. 36, No. 8
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Rapid and Specific Molecular Identification of Methicillin-Resistant Staphylococcus aureus in Endotracheal Aspirates from Mechanically Ventilated Patients

Pascal Vannuffel,1 Pierre-François Laterre,2 Michele Bouyer,3 Jacques Gigi,4 Bernard Vandercam,5 Marc Reynaert,2 and Jean-Luc Gala1,3,*

Laboratory of Applied Molecular Technology,1 and Departments of Intensive Care,2 Microbiology,4 and Internal Medicine,5 St. Luc University Hospital, and Section MSW, Operational Epidemiology and Infectious Diseases, Queen Astrid Military Hospital,3 Brussels, Belgium

Received 24 September 1997/Returned for modification 28 January 1998/Accepted 28 April 1998

Multiplex amplification of femA and mecA genetic determinants allowed an early and rapid identification of methicillin-resistant Staphylococcus aureus (MRSA) in endotracheal aspirates of mechanically ventilated patients. femA and/or mecA amplification and bacteriological results were concordant in 57 of 60 samples. In all three discrepant cases, complementary bacteriological tests confirmed the presence of MRSA first identified by molecular analysis. These results underline the value and rapidity of this molecular diagnosis for MRSA infection and control surveillance in intensive care units. Rapid MRSA detection is expected to have a significant clinical impact not only on patient outcome but also on the costs for isolation and treatment.

* Corresponding author. Mailing address: Laboratory of Applied Molecular Technology, Clos Chapelle aux Champs, 30-UCL/30.46, B-1200, Brussels, Belgium. Phone: 32-2-764 3165. Fax: 32-2-764 3959. E-mail: gala{at}sang.ucl.ac.be.

Journal of Clinical Microbiology, August 1998, p. 2366-2368, Vol. 36, No. 8
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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