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Journal of Clinical Microbiology, August 1998, p. 2371-2374, Vol. 36, No. 8
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Competitive PCR for Quantification of BM5d Proviral DNA in Mice with AIDS

Anna Casabianca, Giuliana Vallanti, and Mauro Magnani*

"Giorgio Fornaini" Institute of Biochemistry, University of Urbino, 61029 Urbino (PS), Italy

Received 17 July 1997/Returned for modification 28 January 1998/Accepted 28 April 1998

Murine AIDS in C57BL/6 mice is caused by a unique mixture of murine leukemia viruses. We report the use of a competitive PCR to detect and quantitate BM5d proviral DNA. This assay allowed discrimination among endogenous wild-type murine retroviruses and BM5d sequences. Furthermore, the method was subsequently used to evaluate the amount of BM5d in infected mice and in infected AZT (zidovudine)-treated mice, providing an effective way to quantitatively evaluate drug efficacy in the murine AIDS model.

* Corresponding author. Mailing address: Istituto di Chimica Biologica "Giorgio Fornaini", Università degli Studi di Urbino, Via Saffi, 2-61029 Urbino, Italy. Phone: 39-722-305211. Fax: 39-722-320188. E-mail: magnani{at}bib.uniurb.it.

Journal of Clinical Microbiology, August 1998, p. 2371-2374, Vol. 36, No. 8
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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Copyright © 1998 by the American Society for Microbiology. All rights reserved.