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Journal of Clinical Microbiology, September 1998, p. 2399-2403, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Differentiation between Mycobacterium tuberculosis and Mycobacterium avium by Amplification of the 16S-23S Ribosomal DNA Spacer

Arunnee Sansila,1 Poonpilas Hongmanee,2 Charoen Chuchottaworn,3 Somsak Rienthong,3 Dhanida Rienthong,3 and Prasit Palittapongarnpim4,*

Department of Pathology, Faculty of Veterinary Medicine, Khonkaen University,1 Department of Pathology, Faculty of Medicine, Ramathibodi Hospital,2 Department of Microbiology, Faculty of Science, Mahidol University,4 and Department of Communicable Disease Control, Ministry of Public Health,3 Bangkok, Thailand

Received 19 December 1997/Returned for modification 15 March 1998/Accepted 6 June 1998

Differentiation between Mycobacterium tuberculosis and M. avium is helpful for the treatment of disseminated mycobacterial infection in AIDS patients. This can traditionally be done by time-consuming biochemical tests or with Accuprobe. Previously, PCR restriction enzyme analysis (PCR-REA) of the 16S-23S rRNA gene spacer was shown to be able to identify a limited number of strains of Mycobacterium. In this study the method was improved by using more specific primers and was tested with 50 clinical isolates of M. tuberculosis and 65 clinical isolates of M. avium complex. Probes specific to the spacers of M. tuberculosis and M. avium were also tested. Both M. tuberculosis and M. avium could be reliably identified either by PCR-REA or by PCR-hybridization, with the results completely agreeing with those obtained by biochemical tests and with the Accuprobe, respectively. The method may therefore be useful as an alternative in-house method for identification of the bacteria.

* Corresponding author. Mailing address: Department of Microbiology, Faculty of Science, Mahidol University, Rama 6 Rd., Bangkok 10400. Phone: 66-2-2461360, ext. 4601. Fax: 66-2-6445411. E-mail: grppl{at}mucc.mahidol.ac.th.

Journal of Clinical Microbiology, September 1998, p. 2399-2403, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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Copyright © 1998 by the American Society for Microbiology. All rights reserved.