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Journal of Clinical Microbiology, September 1998, p. 2471-2476, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Specific Differentiation between
Mycobacterium bovis BCG and Virulent Strains of the
Mycobacterium tuberculosis Complex
Juana
Magdalena,
Philip
Supply, and
Camille
Locht*
Laboratoire de Microbiologie
Génétique et Moléculaire, INSERM U447, Institut
Pasteur de Lille, F-59019 Lille Cedex, France
Received 10 April 1998/Returned for modification 12 May
1998/Accepted 29 May 1998
A PCR procedure based on the intergenic region (IR) separating two
genes encoding a recently identified mycobacterial two-component system, named SenX3-RegX3, was developed and was shown to be suitable for identifying Mycobacterium bovis BCG. The
senX3-regX3 IR contains a novel type of repetitive
sequence, called mycobacterial interspersed repetitive units
(MIRUs). All tested BCG strains exclusively contained 77-bp MIRUs
within the senX3-regX3 IR, whereas all non-BCG
M. tuberculosis complex strains contained a 53-bp MIRU, in
addition to the 77-bp MIRUs. All 148 strains analyzed so far could be
divided into eight different groups according to the copy
numbers of the 77-bp MIRU and to the presence or absence of the 53-bp
MIRU. BCG strains contained either one, two, or three 77-bp MIRUs. The
other strains contained one to five 77-bp MIRUs invariably followed by
a 53-bp MIRU. The consistent absence of the 53-bp MIRU in BCG strains
and its presence in virulent strains allowed us to develop an
enzyme-linked immunosorbent assay using specific capture
oligonucleotide probes to distinguish between BCG and other M. tuberculosis complex strains.
*
Corresponding author. Mailing address: INSERM U447,
Institut Pasteur de Lille, 1, rue du Prof. Calmette, F-59019
Lille Cedex, France. Phone: (33) 3 20 87 11 51. Fax: (33) 3 20 87 11 58. E-mail: camille.locht{at}pasteur-lille.fr.
Journal of Clinical Microbiology, September 1998, p. 2471-2476, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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