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Journal of Clinical Microbiology, September 1998, p. 2522-2529, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Comparison of Amplified Ribosomal DNA Restriction Analysis, Random Amplified Polymorphic DNA Analysis, and Amplified Fragment Length Polymorphism Fingerprinting for Identification of Acinetobacter Genomic Species and Typing of Acinetobacter baumannii

Johannes G. M. Koeleman, Jeroen Stoof, Dennis J. Biesmans, Paul H. M. Savelkoul,* and Christina M. J. E. Vandenbroucke-Grauls

Department of Clinical Microbiology and Infection Control, University Hospital Vrije Universiteit, 1007 MB Amsterdam, The Netherlands

Received 29 January 1998/Returned for modification 12 March 1998/Accepted 21 May 1998

Thirty-one strains of Acinetobacter species, including type strains of the 18 genomic species and 13 clinical isolates, were compared by amplified ribosomal DNA restriction analysis (ARDRA), random amplified polymorphic DNA analysis (RAPD), and amplified fragment length polymorphism (AFLP) fingerprinting. ARDRA, performed with five different enzymes, showed low discriminatory power for differentiating Acinetobacter at the species and strain level. The standardized commercially available RAPD kit clearly enabled the discrimination of all Acinetobacter genomic species but showed great polymorphism between isolates of Acinetobacter baumannii. AFLP fingerprinting with radioactively as well as fluorescently labelled primers showed high discriminatory power for the identification of 18 Acinetobacter genomic species and typing of 13 clinical Acinetobacter isolates. Compared to radioactive AFLP, fluorescent AFLP was technically fast and simple to perform, and it permitted analysis with an automated DNA sequencer. Fluorescent AFLP seems particularly well suited for studying the epidemiology of nosocomial infections and outbreaks caused by Acinetobacter species.

* Corresponding author. Mailing address: Department of Clinical Microbiology and Infection Control, University Hospital Vrije Universiteit, P.O. Box 7057, 1007 MB Amsterdam, The Netherlands. Phone: 31 204440552. Fax: 31 204440473. E-mail: p.savelkoul{at}azvu.nl.

Journal of Clinical Microbiology, September 1998, p. 2522-2529, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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