This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Koeleman, J. G. M.
Right arrow Articles by Vandenbroucke-Grauls, C. M. J. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Koeleman, J. G. M.
Right arrow Articles by Vandenbroucke-Grauls, C. M. J. E.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, September 1998, p. 2522-2529, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Comparison of Amplified Ribosomal DNA Restriction Analysis, Random Amplified Polymorphic DNA Analysis, and Amplified Fragment Length Polymorphism Fingerprinting for Identification of Acinetobacter Genomic Species and Typing of Acinetobacter baumannii

Johannes G. M. Koeleman, Jeroen Stoof, Dennis J. Biesmans, Paul H. M. Savelkoul,* and Christina M. J. E. Vandenbroucke-Grauls

Department of Clinical Microbiology and Infection Control, University Hospital Vrije Universiteit, 1007 MB Amsterdam, The Netherlands

Received 29 January 1998/Returned for modification 12 March 1998/Accepted 21 May 1998

Thirty-one strains of Acinetobacter species, including type strains of the 18 genomic species and 13 clinical isolates, were compared by amplified ribosomal DNA restriction analysis (ARDRA), random amplified polymorphic DNA analysis (RAPD), and amplified fragment length polymorphism (AFLP) fingerprinting. ARDRA, performed with five different enzymes, showed low discriminatory power for differentiating Acinetobacter at the species and strain level. The standardized commercially available RAPD kit clearly enabled the discrimination of all Acinetobacter genomic species but showed great polymorphism between isolates of Acinetobacter baumannii. AFLP fingerprinting with radioactively as well as fluorescently labelled primers showed high discriminatory power for the identification of 18 Acinetobacter genomic species and typing of 13 clinical Acinetobacter isolates. Compared to radioactive AFLP, fluorescent AFLP was technically fast and simple to perform, and it permitted analysis with an automated DNA sequencer. Fluorescent AFLP seems particularly well suited for studying the epidemiology of nosocomial infections and outbreaks caused by Acinetobacter species.

* Corresponding author. Mailing address: Department of Clinical Microbiology and Infection Control, University Hospital Vrije Universiteit, P.O. Box 7057, 1007 MB Amsterdam, The Netherlands. Phone: 31 204440552. Fax: 31 204440473. E-mail: p.savelkoul{at}azvu.nl.

Journal of Clinical Microbiology, September 1998, p. 2522-2529, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Peleg, A. Y., Seifert, H., Paterson, D. L. (2008). Acinetobacter baumannii: Emergence of a Successful Pathogen. Clin. Microbiol. Rev. 21: 538-582 [Abstract] [Full Text]  
  • Deplano, A., De Mendonca, R., De Ryck, R., Struelens, M. J. (2006). External Quality Assessment of Molecular Typing of Staphylococcus aureus Isolates by a Network of Laboratories.. J. Clin. Microbiol. 44: 3236-3244 [Abstract] [Full Text]  
  • Ecker, J. A., Massire, C., Hall, T. A., Ranken, R., Pennella, T.-T. D., Agasino Ivy, C., Blyn, L. B., Hofstadler, S. A., Endy, T. P., Scott, P. T., Lindler, L., Hamilton, T., Gaddy, C., Snow, K., Pe, M., Fishbain, J., Craft, D., Deye, G., Riddell, S., Milstrey, E., Petruccelli, B., Brisse, S., Harpin, V., Schink, A., Ecker, D. J., Sampath, R., Eshoo, M. W. (2006). Identification of Acinetobacter Species and Genotyping of Acinetobacter baumannii by Multilocus PCR and Mass Spectrometry.. J. Clin. Microbiol. 44: 2921-2932 [Abstract] [Full Text]  
  • Beggs, C. B., Kerr, K. G., Snelling, A. M., Sleigh, P. A. (2006). Acinetobacter spp. and the Clinical Environment. Indoor and Built Environment 15: 19-24 [Abstract]  
  • Chang, H. C., Wei, Y. F., Dijkshoorn, L., Vaneechoutte, M., Tang, C. T., Chang, T. C. (2005). Species-Level Identification of Isolates of the Acinetobacter calcoaceticus-Acinetobacter baumannii Complex by Sequence Analysis of the 16S-23S rRNA Gene Spacer Region. J. Clin. Microbiol. 43: 1632-1639 [Abstract] [Full Text]  
  • van der Sar, A. M., Abdallah, A. M., Sparrius, M., Reinders, E., Vandenbroucke-Grauls, C. M. J. E., Bitter, W. (2004). Mycobacterium marinum Strains Can Be Divided into Two Distinct Types Based on Genetic Diversity and Virulence. Infect. Immun. 72: 6306-6312 [Abstract] [Full Text]  
  • Cartelle, M., del Mar Tomas, M., Pertega, S., Beceiro, A., Dominguez, M. A., Velasco, D., Molina, F., Villanueva, R., Bou, G. (2004). Risk Factors for Colonization and Infection in a Hospital Outbreak Caused by a Strain of Klebsiella pneumoniae with Reduced Susceptibility to Expanded-Spectrum Cephalosporins. J. Clin. Microbiol. 42: 4242-4249 [Abstract] [Full Text]  
  • Huber, B. S., Allred, D. V., Carmen, J. C., Frame, D. D., Whiting, D. G., Cryan, J. R., Olson, T. R., Jackson, P. J., Hill, K., Laker, M. T., Robison, R. A. (2002). Random Amplified Polymorphic DNA and Amplified Fragment Length Polymorphism Analyses of Pasteurella multocida Isolates from Fatal Fowl Cholera Infections. J. Clin. Microbiol. 40: 2163-2168 [Abstract] [Full Text]  
  • Lan, R., Reeves, P. R. (2002). Pandemic Spread of Cholera: Genetic Diversity and Relationships within the Seventh Pandemic Clone of Vibrio cholerae Determined by Amplified Fragment Length Polymorphism. J. Clin. Microbiol. 40: 172-181 [Abstract] [Full Text]  
  • Spaargaren, J., Stoof, J., Fennema, H., Coutinho, R., Savelkoul, P. (2001). Amplified Fragment Length Polymorphism Fingerprinting for Identification of a Core Group of Neisseria gonorrhoeae Transmitters in the Population Attending a Clinic for Treatment of Sexually Transmitted Diseases in Amsterdam, The Netherlands. J. Clin. Microbiol. 39: 2335-2337 [Abstract] [Full Text]  
  • Koeleman, J. G. M., Stoof, J., Van Der Bijl, M. W., Vandenbroucke-Grauls, C. M. J. E., Savelkoul, P. H. M. (2001). Identification of Epidemic Strains of Acinetobacter baumannii by Integrase Gene PCR. J. Clin. Microbiol. 39: 8-13 [Abstract] [Full Text]  
  • Antonishyn, N. A., McDonald, R. R., Chan, E. L., Horsman, G., Woodmansee, C. E., Falk, P. S., Mayhall, C. G. (2000). Evaluation of Fluorescence-Based Amplified Fragment Length Polymorphism Analysis for Molecular Typing in Hospital Epidemiology: Comparison with Pulsed-Field Gel Electrophoresis for Typing Strains of Vancomycin-Resistant Enterococcus faecium. J. Clin. Microbiol. 38: 4058-4065 [Abstract] [Full Text]  
  • Morré, S. A., Ossewaarde, J. M., Savelkoul, P. H. M., Stoof, J., Meijer, C. J. L. M., van den Brule, A. J. C. (2000). Analysis of Genetic Heterogeneity in Chlamydia trachomatis Clinical Isolates of Serovars D, E, and F by Amplified Fragment Length Polymorphism. J. Clin. Microbiol. 38: 3463-3466 [Abstract] [Full Text]  
  • Yuan, M., Hall, L. M. C., Savelkoul, P. H. M., Vandenbroucke-Grauls, C. M. J. E., Livermore, D. M. (2000). SHV-13, a Novel Extended-Spectrum beta -Lactamase, in Klebsiella pneumoniae Isolates from Patients in an Intensive Care Unit in Amsterdam. Antimicrob. Agents Chemother. 44: 1081-1084 [Abstract] [Full Text]  
  • Speijer, H., Savelkoul, P. H. M., Bonten, M. J., Stobberingh, E. E., Tjhie, J. H. T. (1999). Application of Different Genotyping Methods for Pseudomonas aeruginosa in a Setting of Endemicity in an Intensive Care Unit. J. Clin. Microbiol. 37: 3654-3661 [Abstract] [Full Text]  
  • Savelkoul, P. H. M., Aarts, H. J. M., de Haas, J., Dijkshoorn, L., Duim, B., Otsen, M., Rademaker, J. L. W., Schouls, L., Lenstra, J. A. (1999). Amplified-Fragment Length Polymorphism Analysis: the State of an Art. J. Clin. Microbiol. 37: 3083-3091 [Full Text]  
  • Vaneechoutte, M., Vauterin, L., van Harsselaar, B., Dijkshoorn, L., De Vos;, P., Koeleman, J. G. M., Stoof, J., Biesmans, D. J., Savelkoul, P. H. M., Vandenbroucke-Grauls, C. M. J. E. (1999). Considerations in Evaluation of the Applicability of DNA Fingerprinting Techniques for Species Differentiation. J. Clin. Microbiol. 37: 3428-3429 [Full Text]  
  • Geornaras, I., Kunene, N. F., von Holy, A., Hastings, J. W. (1999). Amplified Fragment Length Polymorphism Fingerprinting of Pseudomonas Strains from a Poultry Processing Plant. Appl. Environ. Microbiol. 65: 3828-3833 [Abstract] [Full Text]  
  • Meijer, A., Morré, S. A., Van Den Brule, A. J. C., Savelkoul, P. H. M., Ossewaarde, J. M. (1999). Genomic Relatedness of Chlamydia Isolates Determined by Amplified Fragment Length Polymorphism Analysis. J. Bacteriol. 181: 4469-4475 [Abstract] [Full Text]  
  • van Doorn, N. E. M., Namavar, F., Sparrius, M., Stoof, J., van Rees, E. P., van Doorn, L.-J., Vandenbroucke-Grauls, C. M. J. E. (1999). Helicobacter pylori-Associated Gastritis in Mice is Host and Strain Specific. Infect. Immun. 67: 3040-3046 [Abstract] [Full Text]  
  • Olive, D. M., Bean, P. (1999). Principles and Applications of Methods for DNA-Based Typing of Microbial Organisms. J. Clin. Microbiol. 37: 1661-1669 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»