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Journal of Clinical Microbiology, September 1998, p. 2535-2541, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Detection of a Novel Strain of Porcine Circovirus in Pigs with Postweaning Multisystemic Wasting Syndrome

Igor Morozov,1 Theerapol Sirinarumitr,1,2 Steven D. Sorden,3 Patrick G. Halbur,3 Marsha K. Morgan,1 Kyoung-Jin Yoon,3 and Prem S. Paul1,4,*

Veterinary Medical Research Institute,1 Department of Veterinary Pathology,2 Department of Veterinary Diagnostic and Production Animal Medicine,3 and Department of Microbiology, Immunology and Preventive Medicine,4 College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011

Received 12 January 1998/Returned for modification 23 April 1998/Accepted 4 June 1998

Swine infectious agents, especially viruses, are potential public health risks associated with the use of pig organs for xenotransplantation in humans. Therefore, there is a need for better characterization of swine viruses and for the development of diagnostic tests for their detection. We report here isolation of a novel strain of porcine circovirus (PCV) from pigs with postweaning multisystemic wasting syndrome (PMWS). Affected pigs exhibited severe interstitial pneumonia and lymphoid depletion. The complete nucleotide sequence (1,768 nucleotides) of the genome of the PCV isolate was determined and compared with the sequence of the PCV strain isolated from PK-15 cells. Sequence comparison revealed significant differences between the two PCV strains, with an overall DNA homology of 76%. Two major open reading frames (ORFs) were identified. ORF1 was more conserved between the two strains, with 83% nucleotide homology and 86% amino acid homology. ORF2 was more variable, with nucleotide homology of 67% and amino acid homology of 65%. PCR and in situ hybridization demonstrated abundant viral DNA in various organs of pigs with PMWS. In situ hybridization demonstrated that this strain of PCV targets multiple organs and infects macrophages, lymphocytes, endothelial cells, and epithelial cells.

* Corresponding author. Mailing address: Veterinary Medical Research Institute, College of Veterinary Medicine, Iowa State University, Ames, IA 50011. Phone: (515) 294-0913. Fax: (515) 294-1401. E-mail: pspaul{at}iastate.edu.

Journal of Clinical Microbiology, September 1998, p. 2535-2541, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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