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Journal of Clinical Microbiology, September 1998, p. 2565-2570, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Microaerophilic Conditions Promote Growth of Mycobacterium genavense

L. Realini,1,* K. De Ridder,1 J.-C. Palomino,1 B. Hirschel,2 and F. Portaels1

Mycobacteriology Unit, Department of Microbiology, Institute of Tropical Medicine, Antwerp, Belgium,1 and Division of Infectious Diseases, Hôpital Cantonal, Geneva, Switzerland2

Received 10 March 1998/Returned for modification 30 May 1998/Accepted 10 June 1998

Our studies show that microaerophilic conditions promote the growth of Mycobacterium genavense in semisolid medium. The growth of M. genavense at 2.5 or 5% oxygen was superior to that obtained at 21% oxygen in BACTEC primary cultures (Middlebrook 7H12, pH 6.0, without additives). By using nondecontaminated specimens, it was possible to detect growth with very small inocula (25 bacilli/ml) of 12 different M. genavense strains (from nude mice) within 6 weeks of incubation under low oxygen tension; conversely, with 21% oxygen, no growth of 8 of 12 (66.7%) M. genavense strains was detected (growth index, <10). The same beneficial effect of 2.5 or 5% oxygen was observed in primary cultures of a decontaminated clinical specimen. Low oxygen tension (2.5 or 5%) is recommended for the primary isolation of M. genavense. Microaerophilic cultivation of other atypical mycobacteria, especially slow-growing (e.g., Mycobacterium avium) and difficult-to-grow (e.g., Mycobacterium ulcerans) species, is discussed.

* Corresponding author. Mailing address: Institute of Tropical Medicine, Department of Microbiology, Nationalestraat 155, B-2000 Antwerp, Belgium. Phone: 32 3 247 63 24. Fax: 32 3 247 63 33. E-mail: realini{at}microbiol.itg.be.

Journal of Clinical Microbiology, September 1998, p. 2565-2570, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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