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Journal of Clinical Microbiology, September 1998, p. 2590-2596, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Intercontinental Spread of a Multidrug-Resistant MethicillinResistant Staphylococcus aureus Clone

M. Aires de Sousa,1 I. Santos Sanches,1,2 M. L. Ferro,3 M. J. Vaz,4 Z. Saraiva,5 T. Tendeiro,5 J. Serra,5 and H. de Lencastre1,2,6,*

Unidade de Genética Molecular, Instituto de Tecnologia Química e Biológica da Universidade Nova de Lisboa (ITQB/UNL), Oeiras,1 Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa (FCT/UNL), Monte da Caparica,2 Hospital de São João, Oporto,4 and Hospital de São José3 and Instituto Português de Oncologia de Francisco Gentil,5 Lisbon, Portugal, and Laboratory of Microbiology, The Rockefeller University, New York, New York6

Received 6 April 1998/Returned for modification 15 May 1998/Accepted 28 May 1998

Two hundred ten methicillin-resistant Staphylococcus aureus (MRSA) isolates recovered between 1990 and 1997 from three Portuguese hospitals located in Lisbon and Oporto were analyzed by molecular fingerprinting techniques. The hybridization of ClaI restriction digests with the mecA- and Tn554-specific DNA probes combined with pulsed-field gel electrophoresis documented the abrupt appearance and extensive intrahospital spread of the Brazilian epidemic MRSA clone in the 1995 samples of each one of the three hospitals analyzed---suggesting the intercontinental transfer of this strain from Brazil to Portugal. The appearance of this clone may challenge the dominance of another highly epidemic imported clone---the Iberian MRSA, currently the most widely spread MRSA clone in Portuguese hospitals.

* Corresponding author. Mailing address: The Rockefeller University, 1230 York Ave., New York, NY 10021. Phone: (212) 327-8278. Fax: (212) 327-8688. E-mail: lencash{at}rockvax.rockefeller.edu.

Journal of Clinical Microbiology, September 1998, p. 2590-2596, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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