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Journal of Clinical Microbiology, September 1998, p. 2666-2670, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Performance of Transcription-Mediated Amplification and Ligase Chain Reaction Assays for Detection of Chlamydial Infection in Urogenital Samples Obtained by Invasive and Noninvasive Methods

Angelika Stary,1,* Elisabeth Schuh,1 Martina Kerschbaumer,1 Birgit Götz,1 and Helen Lee2

Outpatients' Centre for Diagnosis of Infectious Venero-Dermatological Diseases, Vienna, Austria,1 and Department of Haematology, University of Cambridge, Cambridge, United Kingdom2

Received 16 December 1997/Returned for modification 2 February 1998/Accepted 17 June 1998

Based on the amplification of chlamydia-specific rRNA sequences and the ligase chain reaction (LCR), the performance characteristics of the Gen-Probe Chlamydia trachomatis transcription-mediated amplification (TMA) assay were evaluated with endocervical, urine, and vulval specimens from women and urethral and urine specimens from men and were compared with those for cultures on endocervical, vulval, and urethral swabs. Of the 308 women and 240 men tested, 25 (8.1%) and 44 (18.3%), respectively, were shown to be infected. By using the infected individual as the expanded "gold standard" for calculations, the TMA assay and LCR gave similar performances for the sensitivity of male urethral (93.2%) and urine (88.6 and 86.4%) samples, while culture detected only half of the 44 infected men. In women, the sensitivities of the TMA assay for endocervical and vulval samples were 88 and 92%, respectively, compared to values of 92% for the LCR on both sample types and of 52 and 8%, respectively, for culture. By using first-void urine for chlamydial diagnosis in women, LCR detected 24 (96%) and TMA assay detected 19 (76%) infected individuals, showing a significantly lower sensitivity for urine in women (P = 0.0253). The results indicate a high overall agreement for both amplifying techniques for all examined specimen types, except for female urine. Furthermore, they confirm the previous observation that vulval swabs are an effective alternative noninvasive sample type for the detection of C. trachomatis infection in women by nucleic acid-based amplification technologies.

* Corresponding author. Mailing address: Outpatients' Center for Diagnosis of Infectious Venero-Dermatological Diseases, Franz Jonas-Platz 8/2/3/, A-1210 Vienna, Austria. Phone: 43 1 2707660. Fax: 43 1 27076609. E-mail: angelika.stary{at}univie.ac.at.

Journal of Clinical Microbiology, September 1998, p. 2666-2670, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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