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Journal of Clinical Microbiology, September 1998, p. 2718-2722, Vol. 36, No. 9
Servei de Microbiologia, Hospital
Universitari Germans Trias i Pujol, Facultat de Medicina de la
Universitat Autònoma de Barcelona, Barcelona, Spain
Received 19 February 1998/Returned for modification 31 March
1998/Accepted 15 June 1998
We evaluated a newly commercial enzyme immunoassay (EIA) (Biotest
Legionella Urin Antigen EIA; Biotest AG, Dreieich, Germany) for detection of antigens of all Legionella pneumophila
serogroups with a relatively wide spectrum of cross-reactivity as well
as antigens of other Legionella spp. by comparing its
sensitivity and specificity with those of an EIA for detection of
L. pneumophila serogroup 1 antigen
(Legionella urinary antigen EIA; Binax, Portland, Maine).
Both tests were performed with both concentrated and nonconcentrated urine samples. We also evaluated the capabilities of both EIAs to detect extracted soluble antigens of American Type
Culture Collection (ATCC) Legionella strains
(L. pneumophila serogroups 1 to 14, L. bozemanii, and L. longbeachae). The sensitivity
of the Biotest EIA was 66.66% in nonconcentrated urine and 86.66% in
concentrated urine. The sensitivity of the Binax EIA was 63.76% and
88.88% in nonconcentrated and concentrated urine, respectively. The
specificity was 100% in nonconcentrated and concentrated urine for
both assays. The Binax EIA and Biotest EIA detected extracted soluble antigens of L. pneumophila serogroups 1 to 14 and L. bozemanii ATCC strains. The cross-reactions
observed with the Binax EIA were probably due to common epitopes
directly related to lipopolysaccharide. Further
studies are required to determine the usefulness of the Binax EIA
for detection of urinary antigens from
Legionella species and serogroups other than L. pneumophila serogroup 1. The Biotest EIA proved to
be as rapid, sensitive, and specific as the Binax EIA for the diagnosis
of legionellosis. Concentration of antigen present in urine
increased the sensitivities of both techniques with no reduction in
specificity.
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Comparison of the Binax Legionella Urinary Antigen
Enzyme Immunoassay (EIA) with the Biotest Legionella Urin
Antigen EIA for Detection of Legionella Antigen in both
Concentrated and Nonconcentrated Urine Samples
*
Corresponding author. Mailing address: Servei de
Microbiologia, Hospital Universitari Germans Trias i Pujol, Ctra.
Canyet s/n, 08916 Badalona, Barcelona, Spain. Phone: 34-3-4651200, ext. 474. Fax: 34-3-4657019. E-mail:
jadoming{at}ns.hugtip.scs.es.
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