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Journal of Clinical Microbiology, September 1998, p. 2737-2741, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Molecular Procedure for Rapid Detection of Burkholderia mallei and Burkholderia pseudomallei

Adolf Bauernfeind,* Carsten Roller, Detlef Meyer, Renate Jungwirth, and Ines Schneider

Max von Pettenkofer Institut, D-80336 Munich, Germany

Received 10 November 1997/Returned for modification 21 March 1998/Accepted 11 June 1998

A PCR procedure for the discrimination of Burkholderia mallei and Burkholderia pseudomallei was developed. It is based on the nucleotide difference T 2143 C (T versus C at position 2143) between B. mallei and B. pseudomallei detected in the 23S rDNA sequences. In comparison with conventional methods the procedure allows more rapid identification at reduced risk for infection of laboratory personnel.

* Corresponding author. Mailing address: Max von Pettenkofer Institut, Pettenkoferstr. 9a, D-80336 Munich, Germany. Phone: 0049-89-5160-5268. Fax: 0049-89-5160-5266. E-mail: Adolf.Bauernfeind{at}mvp-bak.med.uni-muenchen.de.

Journal of Clinical Microbiology, September 1998, p. 2737-2741, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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