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Journal of Clinical Microbiology, September 1998, p. 2742-2744, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Identification of Trichosporon asahii by PCR Based on Sequences of the Internal Transcribed Spacer Regions

Takashi Sugita, Akemi Nishikawa,dagger and Takako Shinoda*

Department of Microbiology, Meiji College of Pharmacy, Tanashi, Tokyo, Japan

Received 22 December 1997/Returned for modification 29 April 1998/Accepted 8 June 1998

Trichosporon asahii is a major causative agent of deep-seated trichosporonosis, which has a high mortality rate. To detect T. asahii, we have developed specific oligonucleotide primers based on the internal transcribed spacer regions of this organism's genome. Amplification products were selectively obtained from only T. asahii DNA; the DNAs of other Trichosporon species, as well as those of medically relevant yeasts such as Candida albicans, Cryptococcus neoformans, and Malassezia furfur, were not amplified. This detection system will be useful as a microbiological tool for the diagnosis of trichosporonosis.

* Corresponding author. Mailing address: Department of Microbiology, Meiji College of Pharmacy, 1-22-1 Yato-cho, Tanashi, Tokyo 188-0001, Japan. Phone: 81-424-21-0339. Fax: 81-424-21-1489. E-mail: shinoda{at}my-pharm.ac.jp.

dagger Present address: Department of Immunobiology, Meiji College of Pharmacy, Tanashi, Tokyo, Japan.

Journal of Clinical Microbiology, September 1998, p. 2742-2744, Vol. 36, No. 9
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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